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شناسایی قارچ‌هایی از تیره Didymellaceae همراه علائم مختلف بیماری در برخی گیاهان دارویی در استان خوزستان

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

چکیده

اکالیپتوس (Eucalyptus sp..)، بارهنگ (Plantago sp. )، خارشتر (Alhagi sp..)، سه‏پستان (Cordia myxa)، شیرین‏بیان (Glycyrrhiza glabra)، شوید (Anethum graveolens)، رزماری (Rosmarinus officinalis) و هندوانه ابوجهل (Citrullus colocynthis) از گیاهان دارویی در مناطق مختلف استان خوزستان هستند که می‌توانند میزبان تعداد زیادی قارچ‏های بیماری‌زای و غیر بیماری‏زای گیاهی باشند. در این مطالعه درطول سال‌های 98-1397، 30 نمونه از گیاهان دارویی فوق با علائم لکه‏برگی، شانکر و نکروز ساقه جمع‏آوری و 23 جدایه از شش گونه در تیره Didymellaceae شناسایی شدند. ویژگی-‏های ریخت‌شناسی روی محیط کشت سیب‌زمینی - دکستروز- آگار (PDA) بررسی شد. برای شناسایی مولکولی، بخش‌هایی از ناحیه‏های ITS (بعضی جدایه‏ها)، tub2 (تمام جدایه‏ها) و rpb2 (بعضی‏جدایه‏ها) با استفاده از آغازگرهای مناسب تکثیر و توالی‌یابی شدند. بر اساس تبارزایی مولکولی و ریخت‏شناسی، گونه‏های Allophoma labilis، Didymella glomerata، Epicoccum italicum، Neodidymelliopsis farokhinejad، Nothophoma raii و Xenodidymella glycyrrhizicola شناسایی شدند. براساس دانش ما، این اولین گزارش گونه‌های فوق روی میزبان‏های ذکر شده و اولین ثبت از گونه‏های Epicoccum italicum و . N. raii برای میکوبیوتای ایران است.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of Didymellaceae fungi associated with different disease symptoms in some medicinal plants of Khuzestan Province, Iran

نویسندگان [English]

  • Fatemeh Keshavarzi
  • Reza Farrokhinejad
  • Mehdi Mehrabi-Koushki
Department of Plant protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz
چکیده [English]

Bitter apple (Citrullus colocynthis), camelthorn (Alhagi sp.), cordia (Cordia myxa), dill (Anethum graveolens), eucalyptus (Eucalyptus sp.), licorice (Glycyrrhiza glabra), plantain (Plantago sp.), and rosemary (Rosmarinus officinalis) are medicinal plants distributed in different parts of Khuzestan province. These plants could be host to many pathogenic and non-pathogenic fungi. In this study, 30 samples from medicinal plants showing leaf spot, stem canker and stem necrosis were collected during 2019-2020, and in which 23 isolates of six species in the family Didymellaceae were identified. Molphological characteristics were studied on potato – dextrose - agar (PDA). For molecular study, partial regions of tub2 gene were amplified using appropriate primers and sequenced. Based on molecular phylogeny in combination with morphology, the isolates were identified as follow: Allophoma labilis, Didymella glomerata, Epicoccum italicum, Neodidymelliopsis farokhinejad, Nothophoma raii and Xenodidymella glycyrrhizicola. In our knowledge, this is the first report of above species on surveyed hosts and first record of both E. italicum and N. raii for mycobiota of Iran.

کلیدواژه‌ها [English]

  • Molecular phylogeny
  • morphology
  • pycnidium
  • conidium
  • plant host

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دانش گیاهپزشکی ایران
دوره 53، شماره 2
بهمن 1401
صفحه 163-176

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سابقه مقاله

  • تاریخ دریافت: 29 فروردین 1401
  • تاریخ بازنگری: 01 مرداد 1401
  • تاریخ پذیرش: 22 شهریور 1401

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