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ارزیابی تأثیر نانو ذرات نقره در کنترل بیماری پوسیدگی ریشه ناشی از قارچ Fusarium solani f. sp. phaseoli در گیاه لوبیا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتر وﺣﯿﺪ زرﯾﻦ ﻧﯿﺎ اﺳﺘﺎدﯾﺎر داﻧﺸﮑﺪه ﻋﻠﻮم ﮐﺸﺎورزی و ﺻﻨﺎﯾﻊ ﻏﺬاﯾﯽ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ ﻋﻠﻮم وﺗﺤﻘﯿﻘﺎت ﺗﻬﺮان

2 حامد شیخی دانشجوی کارشناسی ارشد بیوتکنولوژی و به نژادی کشاورزی دانشگاه علوم کشاورزی و صنایع غذایی داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ

چکیده

گیاه گلرنگ با نام علمی L. tinctorius Carthamus از خانواده کمپوزیته و با فعالیت آنتی اکسیدانی می باشد. فعالیت ضد قارچی نانوذرات نقره سنتز شده توسط عصاره گل های گلرنگ علیه بیماری پوسیدگی ریشه لوبیا ناشی از قارچ Fusarium solani در شرایط آزمایشگاهی، گلخانه ای و مزرعه ای بررسی شد. سنتز نانوذرات نقره با روش آنالیز طیف سنجی) UV.Vis (تبدیل فوریه مادون قرمز (FTIR)، پراش اشعه ایکس(XRD) و میکروسکوپ الکترونی عبوری (TEM) ارزیابی شد. فاکتور شدت بیماری در هر بوته لوبیا چیتی(Phaseolus vulgaris L. ) مورد بررسی قرار گرفت. نتایج تشکیل نانوذرات نقره با تغییر رنگ محلول نیترات نقره به قهوه ای تیره بعد از اضافه کردن عصاره به محلول نیترات نقره تایید شد. وجود ماکزیمم جذب توسط آنالیز UV-Visدر محدوده 415 نانومتر دلیلی بر سنتز نانوذرات می باشد. میکروسکوپ الکترونی عبوری شکل نانوذرات را کروی نشان داد. آنالیز XRD اندازه متوسط نانوذرات را 20نانومتر نشان داد. در شرایط آزمایشگاهی، با استفاده از تکنیک اختلاط با محیط کشت حداقل غلظت مهارکنندگی رشد (MIC) روی نرخ رشد میسلیومی قارچ غلظت 200 پی پی ام شد. اثرات بازدارندگی نانوذرات در شرایط گلخانه ای غلظت 800 پی پی ام و در شرایط مزرعه ای غلظت 1000 پی پی ام با روش آغشته سازی بذرهای لوبیا با نانو ذرات مطلوب ارزیابی شد. نتایج تجزیه واریانس نشان داد که اثر متقابل بین غلظت نانو ذرات مختلف و تاثیر قارچ عامل بیماری بر روی شاخص های اندازه گیری شده معنی دار است.

کلیدواژه‌ها

عنوان مقاله [English]

Efect of biosynthesized silver nanoparticles on Fusarium solani the cause of root rot bean's disease

نویسندگان [English]

  • vahid zarrinnia 1
  • hamed sheykhi 2
1 Vahid Zarrinnia Assistant Professor, Faculty of Agricultural Sciences and Food Industries, Islamic Azad University, Research Sciences Branch, Tehran
2 Hamed Sheikhi, M.Sc. Student of Biotechnology and Breeding of Agriculture, Faculty of Agricultural Sciences and Food Industries, Islamic Azad University, Tehran Research Sciences Branch
چکیده [English]

The Safflower Carthamus with the scientific name tinctorius Carthamus L. belongs to the composite family and has antioxidant activity.Antifungal activity of silver nanoparticles synthesized by Safflower Carthamus extract was investigated against bean root rot disease caused by Fusarium solani in laboratory, greenhouse and field conditions.The synthesis of silver nanoparticles was evaluated by spectroscopic analysis (UV.Vis), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and transmission electron microscopy (TEM).Factor disease severity were investigated in each pinto bean (Phaseolus vulgaris L.) plant.The results of the formation of silver nanoparticles were confirmed by changing the color of the silver nitrate solution to dark brown after adding the extract to the silver nitrate solution. The existence of the absorption maximum by UV-Vis analysis in the range of 415 nm is a proof of the synthesis of nanoparticles.Transmission electron microscopy showed the shape of nanoparticles to be spherical. XRD analysis showed the average size of nanoparticles to be 20 nanometers.In laboratory conditions, using the technique of mixing with the culture medium, the minimum growth inhibitory concentration (MIC) on the mycelial growth rate of the fungus was 200 ppm. PPM was evaluated by impregnating bean seeds with nanoparticles. The results of analysis of variance showed that the interaction effect between the concentration of different nanoparticles and the effect of the disease-causing fungus on the measured indicators is significant.

کلیدواژه‌ها [English]

  • Green synthesis
  • AgNPs
  • Carthamus tinctorius
  • antifungal activity

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دانش گیاهپزشکی ایران
دوره 53، شماره 2
بهمن 1401
صفحه 193-207

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  • تاریخ دریافت: 07 خرداد 1401
  • تاریخ بازنگری: 14 شهریور 1401
  • تاریخ پذیرش: 28 آبان 1401

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