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شناسایی و بررسی الگوی بیان ژن پروتئین ذخیره‌ای هگزامرین (Hexamerin) در حشرات دیاپوزی و غیر دیاپوزی سن گندم Eurygaster integriceps

نوع مقاله : مقاله پژوهشی

نویسندگان

1 پردیس کشاورزی و منابع طبیعی دانشگاه تهران، دانشکده علوم و مهندسی کشاورزی، گروه گیاه پزشکی بخش حشره شناسی

2 گروه گیاهپزشکی ، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج ، ایران

3 گروه گیاهپزشکی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران ،کرج، ایران.

4 موسسه زیست شناسی تکاملی (Institut de Biologia Evolutiva)، دانشگاه پمپئوفابرا، بارسلونا، اسپانیا

چکیده

دیاپوز یک سازگاری فیزیولوژیکی است که امکان بقای موجود زنده تحت شرایط سخت را فراهم می‌کند. سن گندم Eurygaster integriceps Put. (Heteroptera: Scutellaridae)، آفت کلیدی گندم و جو و حشره‌ای تک نسلی است که چرخه زندگی این آفت دارای دو فاز متفاوت است، فاز تولیدمثلی و دیاپوز مرحله بالغ. در این پژوهش الگوی بیان ژن پروتئین ذخیره‌ای هگزامرین در اجسام چربی حشرات ماده سن گندم، در دو فاز در حال دیاپوز و خارج از دیاپوز، با استفاده از آنالیز کمی Real time PCR بررسی شد. بیان ژن هگزامرین در ماده‌های دیاپوزی سن گندم 4/6 برابر بالاتر از ماده‌های غیر دیاپوزی بود. نتایج پیش‌بینی قرارگیری درون سلولی و سیگنال پپتید نشان می‌دهد که این پروتئین در دسته پروتئین‌های خارج سلولی قرار می‌گیرد. بر اساس نتایج آنالیز فیلوژنتیک و آنالیز هم‌ردیفی توالی آمینو اسیدی ژن هگزامرین در سن گندم و سایر گونه‌های سن متعلق به راسته همی‌پترا، توالی آمینو اسیدی در کلاد متعلق به راسته همی‌پترا قرار گرفت (با عدد بوت استرپ100) و شباهت و نزدیکی بسیار زیادی را به توالی آمینو اسیدی ژن هگزامرین در سن برگی قهوه‌ای Halyomorpha halys نشان داد (شباهت 6/62 درصد). شناسایی ژن هگزامرین به‌عنوان یک شاخص بیوشیمیایی قابل اعتماد در فاز دیاپوز سن گندم می‌تواند یک گزینه مناسب و ارزشمند در جهت انجام مطالعات آتی مرتبط با دیاپوز در سطوح سلولی و ملکولی در این آفت باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Identification and gene expression analysis of Hexamerin storage protein in diapausing and non-diapausing insects of Sunn pest, Eurygaster integriceps

نویسندگان [English]

  • zeynab alborzi 1
  • Ali R. Bandani 2
  • ahmad ashouri 3
  • mdolors piulachs 4
1 Department of Plant Protection, University College of Agricultural and Natural Resources, University of Tehran
2 Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Institute of Evolutionary Biology (CSIC- Pompeu Fabra University), Barcelona, Spain
چکیده [English]

Diapause is a physiological adaptation that allows an organism to survive adverse environmental conditions. Sunn pest, Eurygaster integriceps (Heteroptera: Scutelleridae), is a key pest of wheat and barley and univoltine species and its life cycle has two different phases, reproductive phase, and adult stage diapause. In this study, the gene expression patterns of Hexamerin storage protein were evaluated by quantitative Real-Time PCR assay in diapause and non-diapause female insects, in the Sunn pest’s fat body. The Hexamerin gene expression in diapausing females of sunn pest was 6.4 times higher than in non-diapausing females. The results of subcellular localization and signal peptide prediction suggested that the protein was categorized as an extracellular protein. According to phylogenetic analysis and multiple alignment analysis of the Sunn pest’s Hexamerin amino acid sequence and other bugs belonging to the Hemiptera order, the amino acid sequence was placed at clade belonging to the Hemiptera order (with 100 bootstrap number) and revealed high similarity and closeness to brown marmorated stink bug, Halyomorpha halys Hexamerin gene amino acid sequence (identity 62.6%). Identifying the Hexamerin gene as a reliable biochemical indicator for Sunn pest diapause can be a proper and valuable candidate for further studies at the cellular and molecular levels in this pest.

کلیدواژه‌ها [English]

  • Biochemical indicator
  • Alignment analysis
  • Phylogenetic analysis
  • Signal peptide
  • Diapause

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دانش گیاهپزشکی ایران
دوره 53، شماره 2
بهمن 1401
صفحه 255-271

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  • تاریخ دریافت: 03 آبان 1401
  • تاریخ بازنگری: 25 آذر 1401
  • تاریخ پذیرش: 07 دی 1401

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