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اثر برهمکنش بین گیاه باقلا و باکتری N179 Paenibacillus polymyxa بر شته سیاه باقلا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 فارغ التحصیل دوره دکتری - گرایش قارچ شناسی و بیماری‌های قارچی گیاهان، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران،

چکیده

ایجاد مقاومت در گیاه علیه آفات از روش‌های جاگزین سموم شیمیایی است که می‌تواند جمعیت آفت را زیر سطح زیان اقتصادی نگه دارد. باکتری‌های همزیست خاک‌زی می‌توانند عوامل مفیدی در راستای افزایش مقاومت گیاهان در برابر حشرات گیاه‌خوار باشند. بر این اساس در این پژوهش تاثیر تیمار گیاه باقلا با باکتری Peanibacillus polymyxa N179 روی فراسنجه‌های جدول زندگی شته سیاه باقلا، Aphis fabae Scopoli (Hemiptera: Aphididae) مورد بررسی قرار گرفت. دو تیمار مورد بررسی در این پژوهش، شامل گیاه باقلا تیمار شده با باکتری و گیاه باقلا بدون تیمار باکتری به عنوان شاهد بود. گیاهان باقلای کاشته شده و جمعیت شته سیاه باقلا در شرایط دمایی 4±25 درجه‌ی سلسیوس، رطوبت نسبی 5±50% و دوره‌ی نوری 14 ساعت روشنایی و 10 ساعت تاریکی پرورش داده شدند. سپس سوسپانسیون باکتری Peanibacillus polymyxa ذکر شده به مقدار پنج میلی‌لیتر به وسیله سرنگ استریل، در خاک گلدان‌ها تزریق شد. بین تیمار باکتری و شاهد از نظر نرخ ذاتی افزایش جمعیت تفاوت معنی‌دار مشاهد شد (5/0≥P). به‌طوری که بیش‌ترین میزان این آماره روی تیمار شاهد (01/0± 42/0 در روز) و کم‌ترین آن روی تیمار باکتری P. polymyxa (001/0±23/0 در روز) مشاهده شد. با توجه به نتایج بدست آمده می‌توان اظهار داشت که گیاهان باقلای تیمار شده با باکتری می‌تواند جهت کاهش جمعیت شته سیاه باقلا موثر باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of interaction between faba beanand Paenibacillus polymyxa N179 on black bean aphid

نویسندگان [English]

  • solmaz azimi 1
  • saedeh shahin injar 1
  • Alireza Alizadeh 2
1 Department of Plant protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.
2 assistance professor of Plant protection, Azarbaijan Shahid Madani University, Tabriz, Iran
چکیده [English]

Plants resistance against pests is one of the methods of replacing chemical pesticide that can keep the pest population below the economic injury level. Soil symbiotic bacteria can be useful factors in increasing the resistance of plants against herbivorous insects. The two treatments investigated in this research included the bean plant treated with bacteria and the bean plant without bacteria treatment as a control. The Vicia faba L. and black bean aphid were grown in a growth chamber at 25±4 0C, %50±5 RH and a photopriod of 14L: 10D hours. Then, 5 ml of bacterial suspension was added into the soil of sown mugs with a sterile syringe. These experiments on the effect of two treatments on faba bean showed that different treatments have a significant differences effect on the biological properties of black bean aphid. The highest developmental time (19.78±0.20 days) and the lowest fertility rate were occurred on P. polymyxa. There were significant differences between different treatments in terms of inherent rate of population increase (P≤0.5). The highest rate was occurred in control treatment (0.42±0.01 day-1) and the lowest in combination P. polymyxa (0.23±0.001 day-1). The life expectancy of black bean aphids, which was grown on P. polymyxa treated, was significantly lower than control. According to the results, it can be stated that faba bean plant treated can be effective in reducing the population of the aphids of the bean.

کلیدواژه‌ها [English]

  • Aphid
  • Bacteria
  • Plant resistance
  • Life table

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دانش گیاهپزشکی ایران
دوره 53، شماره 2
بهمن 1401
صفحه 283-294

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  • تاریخ دریافت: 30 آذر 1401
  • تاریخ بازنگری: 09 بهمن 1401
  • تاریخ پذیرش: 24 بهمن 1401

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