نوع مقاله : مقاله پژوهشی
نویسندگان
گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Cucumber (Cucumis sativus L.) is cultivated in a larger area than other pumpkin plants and it is one of most important plants of the Cucurbitaceae family worldwide. Fusarium wilt disease of cucumber is one of the most important diseases of this crop which caused by Fusarium oxysporum f. sp. cucumerinum (FOC). The aim of this research is biological control of fusarium wilt disease of cucumber by using fungal endophytes such as Fomes fomentarius and Coprinopsis urticicola in laboratory, greenhouse and field conditions. The plant pathogenic fungal (FOC) was isolated from infected cucumber plants and was identified as Fusarium oxysporum f. sp. cucumerinum based on morphological characteristics and molecular phylogenetic analysis of the TEF and ITS genomic regions. Both isolates Fomes fomentarius and Coprinopsis urticicola had inhibitory effects on the development of FOC growth in vitro by dual-culture method with inhibition percentage ranging from 71.81% to 66.81%, respectively. Volatile antifungal effects were observed for Fomes fomentarius isolate with 66.21% inhibition, followed by the isolate Coprinopsis urticicola (61.33%). Both isolates of biocontrol fungi were able to reduce the disease severity by 76-88% and 76-82% in greenhouse and field conditions, respectively. In addition, the effect of the biocontrol isolates on the cucumber growth parameters such as the number of main stems, plant height (cm), root fresh and dry weight (gr) were recorded and the results were shown that the treated plants had a significant increase in growth and production compared to the control.
کلیدواژهها [English]
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