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نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه جیرفت

2 مربی، گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه جیرفت

چکیده

القای مقاومت در گیاهان یکی از روش‌های جدید در مدیریت بیماری‌های گیاهی است. بتا آمینوبوتیریک اسید (BABA) از ترکیباتی است که قادر به القای مقاومت در گیاهان است. در این تحقیق القای مقاومت توسط این ترکیب علیه قارچ Fusarium oxysporum f. sp. radices-cucumerinum F42 در گیاه خیار بررسی شده است. این ترکیب قادر به کاهش معناداری در شدت بیماری از طریق القای مقاومت گردید. بررسی کمی بیان ژن‌های pr1 و lox1 با روش QPCR بیانگر افزایش بیان ژن pr1 بود که دلالت بر القای مسیر مقاومت وابسته به سالیسیلیک اسید دارد. بررسی بیان ژن‌های کیتیناز و بتا-1 و 3-گلوکاناز نشان داد که BABA باعث ایجاد حالت آماده‌باش در بیان این ژن‌ها می‌شود که پس از تلقیح بیمارگر بیان این ژن‌ها افزایش معناداری نسبت به شاهد نشان داد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Induced resistance by β-amino butyric acid (BABA) against fusarium stem and root rot of cucumber

نویسندگان [English]

  • Hamidreza Alizadeh 1
  • Khadijeh Salari 2

1 Assistant Professorو Department of Plant Protection, College of Agriculture, University of Jiroft

2 Instructor, Department of Plant Protection, College of Agriculture, University of Jiroft

چکیده [English]

 One of the new methods in plant disease management is induced resistance. β- amino butyric acid (BABA) is a compounds that able to induce resistance in plants. In this study, induction of resistance by this compound against Fusarium oxysporum f. sp. radices-cucumerinum F42 on cucumber plants was studied. This compound significantly reduced severity of the disease through induced resistance. Quantitative analysis of the expression of LOX1and PR1 genes using QPCR was showed that PR1 gene was up regulated after challenging with pathogen in pretreated plants with BABA. This finding suggests the pathway implicated in the induction of resistance is dependent on salicylic acid. Evaluation of the expression of chitinase and beta-1, 3 - glucanase showed that BABA potentiated the expression of these genes upon challenging with pathogen.

کلیدواژه‌ها [English]

  • chitinase
  • Glucanase
  • Priming
  • Salicylic Acid
  • systemic resistance

 

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