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نقش مهارکنندۀ پای‌پرونیل بوتوکساید در میزان فعالیت ویژۀ آنزیم گلوتاتیون اس‌ترانسفراز پسیل معمولی پسته، Agonoscena pistaciae

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه ولی عصر(عج) رفسنجان

2 استادیار گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه ولی عصر(عج) رفسنجان

3 دانشیار گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه گیلان

چکیده

گلوتاتیون اس‌ترانسفرازها نقش بسیار مهمی در غیرسمی کردن ترکیبات آفت‌کش و مقاومت حشرات در برابر حشره‌کش‌ها ایفا می‌کنند. در این پژوهش تفاوت فعالیت آنزیم‌های گلوتاتیون اس‌ترانسفراز در دو فرم تابستانه و زمستانه پسیل بررسی شده است و اثر غلظت‌های‌ مختلف پی‌پرونیل بوتوکساید (PBO) (5/0، 1، 2، 4 و 8 میلی‌گرم بر لیتر) روی حشرات کامل پسیل پسته در میزان مهار فعالیت این آنزیم با استفاده از سوبسترای 1، کلرو 2 و 4 دی‌نیترو بنزن ((CDNB انجام گرفته است. همچنین میزان فعالیت آنزیم گلوتاتیون اس‌ترانسفراز در زمان‌های 0، 1، 2، 4 و 6 ساعت پس از تیمار حشرات کامل با دزی که بیشترین مهارکنندگی را روی این آنزیم داشت، سنجش شده است. نتایج نشان داد که میزان فعالیت آنزیم در فرم‌های مختلف پسیل با هم ختلاف معنا‌داری (05/0< P) نداشتند و کمترین میزان فعالیت آنزیم گلوتاتیون اس‌ترانسفراز، یا به عبارتی بیشترین میزان مهارکنندگی توسط پای‌پرونیل بوتوکساید در غلظت 5/0میلی‌گرم بر لیتر دیده شد و بیشترین مهارکنندگی در زمان 4 ساعت بعد از کاربرد سینرژیست (PBO) صورت گرفت. با توجه به نتایج این پژوهش چنین به نظر می‌رسد که بتوان با کاربرد مناسب دز PBO، آنزیم گلوتاتیون اس‌ترانسفراز را مهار و با در نظر گرفتن زمان حداکثر مهار توسط این سینرژیست در مدیریت مقاومت پسیل به آفت‌کش‌ها سود برد.

کلیدواژه‌ها


عنوان مقاله [English]

پسیل پسته، سینژیست، مدیریت مقاومت به آفت‌کش‌ها، PBO.

نویسندگان [English]

  • Pooyan Moslehi 1
  • Ali Alizadeh 2
  • Mohammad Ghadamyari 3
1 M. Sc. Student, Department of Crop Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran
2 Assistant Professor, Department of Crop Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran
3 Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Gilan, Iran
چکیده [English]

Glutathion S-transferase enzymes have an important role in the detoxification of pesticides compounds and the resistance of insects to insecticides. In this study, the activity of glutathione S-transferase enzyme between summer and winter forms of pistachio psyllid, were measured and the effect of different concentrations of PBO (0.5, 1,2,4 and 8 mg/Lit) on the inhibition of activity of this enzyme were investigated by using of 1- chloro- 2,4- dinitrobenzene (CDNB) as a substrate. In addition, the activity of glutathione S-transferase were determined at 0, 1, 2, 4 and 6 hour after treatment the adults by the concentration of PBO that had maximum inhibition on this enzyme.  The results indicated that there is no significant deference between the activity of enzyme in different forms of pistachio psyllid (P<0.05) and the lowest activity of glutathione S-transferase or highest inhibition were obtained in 0.5 mg L-1 of  PBO and the maximum inhibition were done at 4 hour after the synergism application.  According to these results, it seems that by using the suitable dose of PBO and matching the time that the synergist has maximum inhibition to the glutathione S-transferase we will be able to promote the resistance management of pistachio psyllid to pesticides.
 

کلیدواژه‌ها [English]

  • PBO
  • Pesticide Resistance management
  • Pistachio psyllid
  • Synergist
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