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بررسی حضور ژن حساسیت Tsn1 در تعدادی از لاین‌های گندم و واکنش آنها نسبت به تزریق توکسین ToxA تولیدشده توسطPyrenophora tritici-repentis

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار مؤسسۀ تحقیقات اصلاح و تهیۀ نهال و بذر، بخش تحقیقات غلات، کرج

2 دانشجوی سابق کارشناسی ارشد، دانشگاه آزاد اسلامی واحد ورامین- پیشوا، دانشکدۀ کشاورزی

3 استاد دانشگاه آزاد اسلامی واحد ورامین- پیشوا، دانشکدۀ کشاورزی

چکیده

لکۀ خرمایی گندم که توسط قارچ Pyrenophora tritici-repentis ایجاد می‌گردد، به عنوان یک بیماری مهم گندم همه ساله خسارات فراوانی به بار می‌آورد. استفاده از تکنیک‌های دقیق، سریع و کم‌هزینه برای ارزیابی لاین‌های گندم نسبت به بیماری‌های مهم همواره مورد توجه به‌نژادگران بوده است. در این تحقیق واکنش 20 لاین گندم نسبت به ToxA که مهم‌ترین فاکتور بیماری‌زایی P. tritici-repentisاست، با استفاده از تزریق توکسین ارزیابی شد. نتایج نشان داد که 9 لاین به همراه رقم Grandin به عنوان شاهد حساس، علائم نکروز را 48 ساعت پس از تزریق توکسین نشان داد و به عنوان لاین‌های حساس به ToxA و بقیۀ لاین‌ها به عنوان لاین‌های مقاوم به توکسین شناسایی شدند. همچنین آزمون PCR با استفاده از آغازگرهای اختصاصی ژن حساسیت Tsn1 انجام گردید. نتایج نشان داد که لاین‌های حساس قادر به تکثیر باند به اندازۀ bp 380 بودند که بیانگر وجود ژن حساسیت Tsn1 است. همۀ لاین‌های مقاوم در آزمون تزریق توکسین، در آزمون PCR نیز قادر به تکثیر ژن Tsn1 نبودند که مؤید نتایج تزریق توکسین بود. نتایج این پژوهش نشان می‌دهد، از این روش می‌توان برای غربال‌گری مقدماتی لاین‌های گندم به عنوان انتخاب منفی برای شناسایی لاین‌های حساس و حذف آنها از برنامه‌های به‌نژادی استفاده کرد.
 

کلیدواژه‌ها


عنوان مقاله [English]

The presence of the susceptibility gene, Tsn1, in some wheat lines and their reactions to ToxA infiltration produced by Pyrenophora tritici-repentis

نویسندگان [English]

  • Rahim Mehrabi 1
  • Nasim Karami 2
  • Mohammad Torabi 3
1 Assistant professor, Cereal Research Dep., Seed and Plant Improvement Institute, Karaj
2 Former MSc. Student, College of Agriculture, Islamic Azad University, Pishva-Varamin Branch,
3 Professor, College of Agriculture, Islamic Azad University, Pishva-Varamin Branch,
چکیده [English]

The tan spot disease caused by Pyrenophora tritici-repentis is one of the most important diseases of wheat causing significant losses annually. The use of accurate, quick and cost-effective techniques, for evaluation of wheat lines to important diseases have been always considered by wheat breeders. In this study, reactions of 20 wheat lines to ToxA, the most important virulence factor of P. tritici-repentis, were evaluated using toxin infiltration approach. The results showed that 9 lines along with Grandin cv. as the susceptible cultivar, exhibited necrosis symptoms within 48 hours after toxin infiltration and, thus, were identified as the susceptible lines to ToxA. The remaining genotypes were identified as resistant lines to the toxin. In addition, PCR assays were used to detect the susceptibility gene, Tsn1, using gene-specific primers. The results showed that in susceptible lines a 380 bp band could be amplified revealing that Tsn1 gene was presented in these lines. All the resistant lines did not amplify Tsn1 gene and confirmed the results of toxin infiltration. The results of this study showed that this approach can be used for initial screening of wheat lines as a negative selection method for identification and subsequent elimination of susceptible lines from breeding programs.
 

کلیدواژه‌ها [English]

  • ToxA toxin
  • Susceptibility gene
  • Tan spot of wheat
  • Ts1
  • Pyrenophora tritici-repentis
 
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