1دانشجوی کارشناسی ارشد گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
2دانشیار گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
3استاد گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
چکیده
در بررسی سازگاری میسلیومی جدایه‌های قارچ Valsa sordida روی دو نوع محیط کشت سیب‌زمینی- دکستروز-آگار و جو دوسر- آگار، از 91 جدایه که از استان‌های مختلف کشور و از برخی درختان مثمر و غیرمثمر طی سال‌های 1383 تا 1390 جمع‌آوری شده بود، استفاده شد. در الگوی تعیین‌شده، هر جدایه با خود و با دیگر جدایه‌ها تلاقی داده شد. خط واکنش تیره‌رنگ که ناسازگاری بین جدایه‌ها را نشان می‌داد، پس از گذشت هفت روز ایجاد گردید. بر اساس نتایج، 38 گروه سازگار رویشی تک‌عضوی و هشت گروه چندعضوی روی محیط کشت سیب‌زمینی- دکستروز- آگار و 25 گروه سازگار رویشی تک‌عضوی و 10 گروه چندعضوی روی محیط کشت جو دوسر- آگار شناسایی شدند که نشان‌دهندۀ وجود تنوع ژنتیکی زیاد در میان جدایه‌های قارچ بود. در این مطالعه، ارتباطی بین گروه‌های شناسایی‌شده روی هر دو نوع محیط کشت با پراکنش جغرافیایی جدایه‌های قارچی و منشأ میزبانی آنها مشاهده نشد.
Mycelial compatibility of Valsa sordida isolates in Iran
نویسندگان [English]
Sudabeh Bozorgmanesh1؛ Khalil-Berdi Fotouhifar2؛ Mohammad Javan Nik Khah3
1. M.Sc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2Associate Professor University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]
In order to study of mycelial compatibility of Valsa sordida isolates on two culture media including potato dextrose agar and oat meal agar, 91 isolates that were collected from different provinces in 2004 and 2011 were used. In the prepared test, each isolate was paired with itself and with any of the other isolates. Dark barrage zone that shows incompatibility between two isolates, were observed seven days after parings. According to the results, thirty-eight single mycelial compatibility (MC) groups and eight multi-merge groups were identified on PDA culture medium and twenty-five single m-c groups and ten multi-merge groups on OMA culture medium were detected, showing the existence of high genetic diversity among the fungal isolates. In this study, no relationship between obtained MC groups on two culture media with geographic distribution and host plant origins of the fungal isolates was observed.
کلیدواژه‌ها [English]
fungus, Cytospora chrysosperma, Genetic diversity, heterokaryon and barrage zone
مراجع
Adams, G.C., Hammar, S.A. & Proffer, T. (1990). Vegetative compatibility in Leucostoma persoonii. Phytopathology, 80, 287-291.
Aghapour, B. (2010). Study on morphology and genetic diversity of isolates of Valsa sordida, the causal agent of tree canker, in Golestan province using molecular marker, rep-PCR. MSc. Thesis, submitted to University of Tehran, Karaj, Iran, Pp. 112.
Bozorgmanesh, S. (2012). Study on vegetative compatibility and genetic diversity of Valsa sordida isolates using molecular marker, MP-PCR. MSc. thesis submitted to University of Tehran, Karaj, Iran, Pp. 112.
Bozorgmanesh, S., Fotouhifar, Kh. B. & Heidarian, R. (2012). Introduction of some new host plants for Cytospora chrysosperma.20th Iran. Plant Protec. Cong., Shiraz, Iran. p. 455 (Abst.). (in Farsi).
Fotouhifar, Kh. B. (2007). Taxonomic research on Iranian form-species of form-genus Cytospora Ehrenberg. Ph.D. thesis submitted to University of Tehran, Karaj, Iran, Pp. 183.
Fotouhifar, Kh.B., Hedjaroude, G.A., Ershad, D., Moussavi, S.M., Okhovvat, S.M. & Javan-Nikkhah, M. (2007). New information on the form-genus Cytospora in Iran (I). Rostaniha, 8(2), 129-149. (in Farsi)
Glass, N.L. & Kuldau, G.A. (1992). Mating type and vegetative incompatibility in filamentous Ascomycetes. Annu. Rev. Phytopathol., 30, 201-224.
Glass, N.L., Jacobson, D.J. & Shiu, P.K.T. (2000). The genetics of hyphal fusion and vegetative incompatibility in filamentous Ascomycete fungi. Annual Review of Genetics, 34, 165-186.
Hayova, V.P. & Minter, D.V. (1998). Valsa sordida. IMI Descriptions of Fungi and Bacteria. Set 137, No. 1370, CAB International, UK.
Kepley, J.B. & Jacobi, W.R. (2000). Pathogenicity of Cytospora fungi on six hardwood species. Journal of Arboriculture, 26(6), 329-332.
Kull, L.S., Pederson, W.L., Palmquist, D. & Hartan, G.L. (2004). Mycelial compatibility grouping and aggressiveness of Sclerotinia sclerotiorum. Plant Disease, 88, 325-332.
Liu, Y.C. & Milgroom, M.G. (2007). High diversity of vegetative compatibility types in Cryphonectria parasitica in Japan and China. Mycologia, 99(2), 279-284.
Mccallum, B.D., Tekauz, A. & Gilbert, J. (2004). Barrage zone formation between vegetatively incompatible Fusarium graminearum (Gibberella zeae) isolates. Phytopathology, 94(5), 432-437.
Proffer, T.J. & Hart, J.H. (1988). Vegetative compatibility groups in Leucocytospora kunzei. Phytopathology, 78(3), 256-260.
Ploetz, R.C. & Shokes, F.M. (1989). Variability among isolates of Diaporthe phaseolorum f.sp. meridionalis in different vegetative compatibility groups. Canadian Journal of Botany, 67, 2751-2755.
Smit, W.A., Wingfield, B.D. & Wingfield, M. J. (1997). Vegetative incompatibility in Diaporthe ambigua. Plant Pathology, 46, 366-372.
Suzaki, K. (2008). Population structure of Valsa ceratosperma, causal fungus of Valsa canker, in apple and pear orchards. Journal of General Plant Pathology, 74,128-132.
Wang, D., Iezzoni, A. & Adams, G.C. (1998). Genetic heterogeneity of Leucostoma species in Michigan peach orchards. Phytopathology, 88(5), 376-381.
ابتدای صفحه