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بررسی ارتباط برخی از فاکتورهای بیماری‌زایی با توان هستۀ یخ در استرین‌های Pseudomonas syringae جدا‌شده از درختان میوۀ هسته‌دار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد بیماری‌شناسی گیاهی ، گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه فردوسی مشهد

2 دانشیار، گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه فردوسی مشهد

چکیده

پدیدۀ حد نصاب احساس یکی از پدیده‌های کنترل‌کنندۀ تولید فاکتورهای مؤثر در بیماری‌زایی برخی از باکتری‌ها است، اما ارتباط توانایی ایجاد هستۀ یخ در باکتری P. syringaeکه یکی از فاکتورهای مؤثر در تشدید بیماری است، با این پدیده مشخص نشده است. به منظور بررسی آن، در بهار 1392، 248 نمونه (شامل برگ و شکوفه) از سرشاخۀ درختان میوۀ هسته‌دار استان خراسان رضوی، جمع‌آوری شد. باکتری‌های مولد هستۀ یخ، شناسایی شدند و از میان آنها جدایه‌های متعلق به گونۀ P. syringae با استفاده از آزمون‌های لوپات (LOPAT) تشخیص داده شدند. از میان فاکتور‌های مؤثر بر بیماری‌زایی سه فاکتور تحرک، حساسیت به پراکسید هیدروژن و تولید پایووردین بررسی شدند. میزان تحرک در جدایه‌هایی که قدرت هستۀ یخ زیادی داشتند، به‌طور معناداری بیشتر از جدایه‌هایی بود که به عنوان هستۀ یخ ضعیف، شناسایی شدند. اختلاف معناداری در رابطه با حساسیت به پراکسید هیدروژن و تولید پایووردین در میان جدایه‌های قوی و ضعیف هستۀ یخ، مشاهده نشد. هموسرین لاکتون موجود در شش جدایۀ انتخاب‌شده از P. syringae‌ های مربوط به گروه‌های مختلف هستۀ یخ، جداسازی و با استفاده از بیوسنسور Chromobacter violaceum CV026، روی صفحۀ کروماتوگرافی (Thin-layer Chromatography) ردیابی شدند. نتایج ما ارتباط مستقیمی بین قدرت هستۀ یخ و میزان تولید هموسرین لاکتون‌ها را در باکتری P. syringae نشان داد و ثابت کرد جدایه‌هایی که تحرک بیشتری دارند، قدرت هستۀ یخ بیشتری نیز دارند.
 
 

کلیدواژه‌ها

عنوان مقاله [English]

An investigation on the correlation of some virulence factors with ice nucleation activity in Pseudomonas syringae strains isolated from stone fruit trees

نویسندگان [English]

  • Elaheh Taheri 1
  • Saeed Tarighi 2
  • Parissa Taheri 2
1 Former M.Sc. Student , Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Former M.Sc. Student , Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

The quorum sensing is a phenomenon which controls the production of virulence factors in some bacteria. But the correlation between the ice nucleation activities which is one of the most important virulence factor in P.syringae with this system in unknown. In order to investigate that, in spring of 2013, 248 samples (including leaves and flowers) were collected from twigs of stone fruit trees in Khorasan Razavi province. Ice nucleation active bacteria, were identified and among them the P. syringae isolates were distinguished by LOPAT tests. Among virulence factors three of them such as swarming motility, susceptibility to hydrogen peroxide and pyoverdine production were studied. The swarming motility of the isolates with high ice nucleation activity were significantly more than those isolates which indicated low ice nucleation activity. There was no significant different in susceptibility to hydrogen peroxide and pyoverdine production between high and low ice nucleation active isolates. The production of homoserine lactones in six selected P. syringae belonged to the different groups of ice nucleation activity were extracted and detected using Chromobacter violaceum CV026 which was overlaid on Thin-layer chromatography plates. Our results indicated direct correlation between ice nucleation activity and the amount of homoserine lactone production and confirmed that the hypermotile isolates have high ice nucleation activity.
 

کلیدواژه‌ها [English]

  • Bacteria
  • homoserin lactone
  • ice nucleation
  • qourum sensing

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دانش گیاهپزشکی ایران
دوره 46، شماره 2 - شماره پیاپی 2
پاییز و زمستان
مهر 1394
صفحه 351-362

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سابقه مقاله

  • تاریخ دریافت: 24 فروردین 1394
  • تاریخ بازنگری: 24 مرداد 1394
  • تاریخ پذیرش: 27 آبان 1394

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