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غربال سودومونادهای فلورسنت بر پایة تولید سیدروفور و القای تولید اتیلن گیاه برای القای مقاومت سیستمیک علیه بیماری باکتریایی نواری برگ گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار گروه گیاه‌پزشکی، دانشکدة کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 دانشیار، مؤسسة تحقیقات گیاه‌پزشکی کشور، تهران، ایران

3 دانشیار گروه گیاه‌پزشکی، دانشکدة کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

چکیده

در این تحقیق اثر سودومونادهای فلورسنت القاء‌کنندة مقاومت سیستمیک در کنترل بیماری باکتریایی نواری برگ گندم بررسی شد. با توجه به اهمیت سیدروفور (سیدروفور) تولیدشده توسط این باکتری‌ها در القای مقاومت سیستمیک، محیط کشت CAS آگار (chrome azurol S) برای غربال باکتری‌هایی با بیشترین توان تولید سیدروفور استفاده شدند. از بین 200 جدایه که از گندمزار مختلف استان‌های شمال‌غرب کشور جداسازی شدند، 37 جدایه توانایی بالایی در تولید سیدروفور داشتند و هاله‌ای با قطر بیش از 15 میلی‌متر روی محیط CAS ایجاد کردند. این جدایه‌ها با آزمون تولید اتیلن بررسی شدند تا جدایه‌هایی که قابلیت القای مقاومت سیستمیک روی گندم را دارند غربال شوند. از بین این جدایه‌ها تنها چهار جدایه واکنش‌پذیری گیاه نسبت به Xanthomonas translucens pv. cerealis (XTC) را افزایش دادند و غلظت اتیلن تولیدشده در قطعه‌های برگی گیاهان تیمارشده با این جدایه‌ها بیش از 2 پیکومول بر میلی‌لیتر هوا بود. تأثیر این جدایه‌ها روی بیماری نواری برگ گندم در شرایط اتاقک کشت بررسی و مشخص شد که تنها جدایة 58A می‌تواند به‌صورت معنی‌داری این بیماری را کاهش دهد. بررسی تغییرپذیری آنزیمی بوته‌های تیمارشده با این جدایه در طول فرآیند بیماری‌زایی نشان داد که حضور این جدایه در ریشه‌های گندم تأثیری بر میزان فعالیت آنزیم‌های پراکسیداز (PO) و فنیل آلانین آمونیالیاز (PAL) در بخش هوایی آن ندارد، اما پس از مایه‌زنی بیمارگر فعالیت هر دو آنزیم افزایش پیدا کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Screening of fluorescent pseudomonads based on production of siderophore and induction of plant ethylene production for induction of systemic resistance against wheat bacterial leaf streak

نویسندگان [English]

  • Vahid Fallahzadeh-Mamaghani 1
  • Ali Alizadeh Aliabadi 2
  • Akbar Shirzad 3
1 Assistant Professor, Depatrment of Plant Protection, College of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
2 Associate Professor, Iranian Research Institute of Plant Protection, Tehran, Iran
3 Associate Professor, Depatrment of Plant Protection, College of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
چکیده [English]

In this study effect of systemic resistance inducing fluorescent pseudomonads on wheat bacterial leaf streak was investigated. According to the importance of produced siderophore by this kind of bacteria in the induction of systemic resistance, CAS agar medium was used for screening of the isolates with the highest potential for siderophore production.  From the 200 isolates that isolated from North West part of Iran, 37 could produce a large amount of siderophore and the diameter of hallo zones produced by these isolates on CAS medium agar was more than 15 mm. Ethylene production bioassay was used for screening the isolates with the potential for induction of systemic resistance in wheat. Among these isolates, only four increased the plant reaction potential against XTC and concentration of ethylene produced by leaf pieces of plants treated by these isolates was more than 2 pmol/ml air. The effect of selected isolates on bacterial leaf streak of wheat in growth chamber was investigated and it was found that only 58A can significantly decrease this disease. Investigation of the enzymatic changes of plant treated by this isolate during pathogenicity process showed that the presence of this bacterium on the wheat roots doesn’t have any effect on peroxidase (PO) and phenylalanine ammonia lyase (PAL) activity in foliage, but after inoculation of the pathogen, the activity of both of them increased.

کلیدواژه‌ها [English]

  • Ethylene
  • Pseudomonas
  • Xanthomonas
  • Wheat
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