عنوان مقاله [English]
Microbial volatiles have an important role in plant growth promotion and protecting them against plant pathogens. In this work, we evaluated whether B. subtilis volatiles promote plant growth and induce resistance against P. syringae pv. tomato DC3000. Arabidopsis seedlings exposed to bacterial volatiles in two compartments I-plate system. Volatiles increased plant growth significantly compared to control. Exposure of plant with bacteria volatiles reduced disease index from 80% to 40%. Pathogenic bacteria population in plant leaves reached up to 1.1×106 CFU/g aerial part wet weight, in volatile treatment, however pathogen population reached up to 1.3×108 in control plants. Expressions of PR-1, PDF1.2, and ChiB have been evaluated as marker genes for salicylic acid, jasmonic acid and ethylene-depended pathways, respectively. Bacteria volatiles boosted the expression of PR-1 and PDF1.2, significantly. These genes expressed strongly and rapidly which represent plant defense priming by bacteria volatiles. In conclusion, volatiles from B. subtilis GB03 not only improved plant growth significantly but also increased expression of defense-related genes and eventually suppressed disease in Arabidopsis.
Blom, D., Fabbri, C., Eberl, L. & Weisskopf, L. (2011). Volatile-mediated killing of Arabidopsis thaliana by bacteria is mainly due to hydrogen cyanide. Applied Environmental Microbiology, 77, 1000-1008.
Brooks, D. M., Bender, C. L. & Kunkel, B. N. (2005). The Pseudomonas syringae phytotoxin coronatine promotes virulence by overcoming salicylic acid-dependent defences in Arabidopsis thaliana. Molecular Plant Pathology 6, 629-639.
Cho, S. M., Kang, B. R., Han, S. H., Anderson, A. J., Park, J. Y., Lee, Y. H., Cho, B. H., Yang, K. Y., Ryu, C. M. & Kim, Y. C. (2008). 2R, 3R-butanediol, a bacterial volatile produced by Pseudomonas chlororaphis O6, is involved in induction of systemic tolerance to drought in Arabidopsis thaliana. Molecular Plant Microbe Interaction, 21, 1067-1075.
Conrath, U. (2011). Molecular aspects of defence priming. Trends in Plant Science, 16, 524-531.
Conrath, U., Beckers, G. J., Flors, V., Garcia-Agustin, P., Jakab, G., Mauch, F., Newman, M. A., Pieterse, C. M. J., Poinssot, B., Pozo, M. J., Pugin, A., Schaffrath, U., Ton, J., Wendehenne, D., Zimmerli, L. & Mauch-Mani, B. (2006). Priming: getting ready for battle. Molecular Plant Microbe Interaction, 19, 1062-1071.
Conrath, U., Beckers, G.J., Langenbach, C. J. & Jaskiewicz, M. R. (2015). Priming for enhanced defense. Annual Review of Phytopathology, 53, 97-117.
Girón-Calva, P. S., Molina-Torres, J. & Heil, M. (2012). Volatile dose and exposure time impact perception in neighboring plants. Journal of Chemical Ecology, 38, 226-228.
Glazebrook, J. (2005). Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Review of Phytopathology, 43, 205-227.
Hahm, M. S., Sumayo, M., Hwang, Y. J., Jeon, S. A., Park, S. J., Lee, J. Y., Ahn, J. H., Kim, B. S., Ryu, C. M. & Ghim, S. Y. (2012). Biological control and plant growth promoting capacity of rhizobacteria on pepper under greenhouse and field conditions. Journal of Microbiology, 50, 380-385.
Heil, M. (2001). The ecological concept of costs of induced systemic resistance (ISR). European Journal of Plant Pathology, 107, 137-146.
Heil, M. (2002). Ecological costs of induced resistance. Current Opinion in Plant Biology, 5, 345-350.
Kai, M., Haustein, M., Molina, F., Petri, A., Scholz, B. & Piechulla, B. (2009). Bacterial volatiles and their action potential. Applied Microbiology and Biotechnology, 81, 1001-1012.
Kishimoto, K., Matsui, K., Ozawa, R. & Takabayashi, J. (2007). Volatile 1-octen-3-ol induces a defensive response in Arabidopsis thaliana. Journal of General Plant Pathology, 73, 35-37.
Lee, B., Farag, M. A., Park, H. B., Kloepper, J. W., Lee, S. H. & Ryu, C. M. (2012). Induced resistance by a long-chain bacterial volatile: elicitation of plant systemic defense by a C13 volatile produced by Paenibacillus polymyxa. PLoS One 7, e48744.
Newman, M.-A., Sundelin, T., Nielsen, J.T. & Erbs, G. (2013). MAMP (Microbe-Associated Molecular Pattern) triggered immunity in Plants. Frontiers in Plant Science, 4, 1-25.
Ongena, M. & Jacques, P. (2008). Bacillus lipopeptides versatile weapon for plant disease control. Trends in microbiology, 16, 115-125.
Pieterse, C. M., Van Der Does, D., Zamioudis, C., Leon-Reyes, A. & Van Wees, S. C. (2012). Hormonal modulation of plant immunity. Annual Review of Cell and Developmental Biology, 28, 489-521.
Rudrappa, T., Biedrzycki, M. L., Kunjeti, S. G., Donofrio, N. M., Czymmek, K. J., Paul, W. P. & Bais, H. P. (2010). The rhizobacterial elicitor acetoin induces systemic resistance in Arabidopsis thaliana. Communicative and Integrative Biology, 3, 130-138.
Ryu, C. M., Farag, M. A., Hu, C. H., Reddy, M. S., Kloepper, J. W. & Pare, P. W. (2004). Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiology, 134, 1017-1026.
Ryu, C. M., Farag, M. A., Hu, C. H., Reddy, M. S., Wei, H. X., Pare, P. W. & Kloepper, J. W. (2003). Bacterial volatiles promote growth in Arabidopsis. Proceedings of the National Academy of Sciences, 100, 4927-4932.
Schulz, S. & Dickschat, J. S. (2007). Bacterial volatiles: the smell of small organisms. Natural Product Reports, 24, 814-842.
Song, G. C., Choi, H. K. & Ryu, C.-M. (2015). Gaseous 3-pentanol primes plant immunity against a bacterial speck pathogen, Pseudomonas syringae pv. tomato via salicylic acid and jasmonic acid-dependent signaling pathways in Arabidopsis. Frontiers in plant science 6:821. doi: 10.3389/fpls.2015.00821
Thakore, Y. (2006). The biopesticide market for global agricultural use. Industrial Biotechnology Letters 2, 194-208.
Vespermann, A., Kai, M. & Piechulla, B. (2007). Rhizobacterial volatiles affect the growth of fungi and Arabidopsis thaliana. Applied and Environmental Microbiology, 73, 5639-5641.