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بررسی سه جدایۀ ویروس وای سیب‌زمینی جداشده از استان خوزستان بر پایۀ ژن پروتئین پوششی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی دانشگاه ولیعصر(عج) رفسنجان

2 استادیار، دانشکدۀ کشاورزی دانشگاه ولیعصر(عج) رفسنجان

چکیده

ویروس وای سیب‌زمینی (Potato virus Y, PVY) یکی از ویروس­های مهم زیانبار با دامنۀ میزبانی گسترده است. در این پژوهش با توجه به شناسایی ویروس وای سیب‌زمینی و جداسازی آن از کشتزارهای شلغم استان خوزستان در سال زراعی 1392، ویژگی­های سه جدایۀ انتخابی از شهرستان­های دزفول، شوشتر و شوش (7Tu، 9Tu و 10Tu) بررسی شد. در آزمون تعیین دامنۀ میزبانی، سه جدایه روی گیاهانی متعلق به سه خانوادۀ Solanaceae، Chenopodiaceae و Amaranthaceae، به‌صورت مکانیکی مایه­زنی شدند. هر سه جدایه روی سیب‌زمینی، گوجه‌فرنگی، فلفل، تاتوره و گل تکمه‌ای نشانه‌های موضعی و فراگیر (سیستمیک) ایجاد کردند ولی روی بوته‌های سلمه‌تره، نشانه‌هایی مشاهده نشد. در آزمون SDS-PAGE، دو باند با اندازۀ حدود 33 و 31 کیلودالتونی در آمودۀ خالص‌شدۀ هر سه جدایۀ خالص‌سازی‌شده مشاهده شد. برای تعیین سویۀ جدایه‌های انتخابی از جفت آغازگرهای اختصاصی سویۀ PVY (CF/OR, NF/NR, OF/OR و PVY3TIM/PVNIBIP) که بر پایۀ ناحیۀ (CP) طراحی شده‌اند استفاده شد. در آزمون PCR با آغازگرهای اختصاصی سویۀ O (OR/OF) و سویۀ NTN (PVY3TIM وPVNIBIP) به ترتیب دو قطعۀ 608 و 1200 جفت بازی در هر سه جدایه افزایش شد. در ادامه، ناحیۀ CP هر سه جدایه‌، افزایش، همسانه‌سازی و توالی­یابی شد. درنهایت با استفاده از مجموع نتایج به‌دست‌آمده از آزمون­های زیستی (بیولوژیکی) و بررسی توالی نوکلئوتیدی و آمینواسیدی مشخص شد که جدایه‌های 7Tu، 9Tu و 10Tu جداشده از کشتزارهای شلغم استان خوزستان بیشترین همسانی را با سویه‌های NTN و W ویروس PVY دارند. این نخستین گزارش از شناسایی ویروس وای سیب‌زمینی در کشتزارهای شلغم است.

کلیدواژه‌ها

عنوان مقاله [English]

The characterization of three isolates of Potato virus Y from khuzestan province according to the coat protein

نویسندگان [English]

  • Seyed Sajjad Zabihi 1
  • Samin-o-Sadat Hosseini 2
  • Seyed Ahmad Hosseini 2
1 Former M. Sc. Student, Faculty of Agricluture, Vali-e-Asr University of Rafsanjan, Iran
2 Assistant Professor, Faculty of Agricluture, Vali-e-Asr University of Rafsanjan, Iran
چکیده [English]

Potato virus Y is one of the most important damaging viruses with a broad host range. In this research due to detection and isolation of Potato virus Y in turnip fields of Khuzestan province during 2014 growing season, characteristics of three selected isolates (7Tu, 9Tu and 10Tu) from Dezful, Shushtar and Shush cities were investigated. To analyze the host range of the isolates, three selected isolates were mechanically inoculated on some members of Solanaceae, Chenopodiaceae, and Amaranthaceae. All three isolates induced local and systemic symptoms on the leaves of potato, tomato, pepper, datura, and gomphrena plants, but no symptoms were observed on Chenopodium quinoa. Analysis of the PVY partially purified preparations indicated that molecular weight of two protein bands obtained by SDS-PAGE was estimated to be about 33 and 31 KDa. To determine the strain of selective isolates the specific primer pairs CF/OR, NF/NR, OF/OR, and NF/OR were used. Using OF/OR and PVY3TIM/PVNIBIP primers, two fragments with 609 and 1200 bp length respectively were amplified. The CP region in the all three isolates was amplified, cloned and sequenced. The results of all aforementioned tests showed that 7Tu, 9Tu, and 10Tu isolates are probably a member of NTN or W strain groups. According to our knowledge, this is the first report on occurrence of PVY in turnip fields.

کلیدواژه‌ها [English]

  • Coat protein
  • turnip
  • Potato virus Y
  • RT-PCR
  • SDS-PAGE

مراجع

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سابقه مقاله

  • تاریخ دریافت: 18 خرداد 1395
  • تاریخ بازنگری: 04 دی 1395
  • تاریخ پذیرش: 07 اسفند 1395

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