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برهمکنش Pseudomonas fluorescens UTP100، رقم‌های گندم و قارچ بیمارگر Fusarium culmorum

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، پردیس کشاورزی ومنابع طبیعی دانشگاه تهران، کرج

2 دانشیار، پردیس کشاورزی ومنابع طبیعی دانشگاه تهران، کرج

3 دانشجوی دکتری، پردیس کشاورزی ومنابع طبیعی دانشگاه تهران، کرج

چکیده

در این بررسی، تأثیر جدایۀ Pseudomonas fluorescens UTP100 در برهمکنش سه رقم گندم (فلات، مرودشت و نیک­نژاد) و قارچ بیمارگر Fusarium culmorum بررسی شد. نتایج نشان داد، تیمار بذرهای هر سه رقم گندم با ریزوباکتری UTP100 منجر به کاهش معنی­دار درصد آلودگی قارچ بیمارگر می­شود. میزان کلونیزاسیون ریزوباکتری روی ریشۀ سه رقم گندم با یکدیگر متفاوت بود، به‌طوری‌که بیشترین لگاریتم تعداد (شمار) سلول باکتری در میلی­گرم ریشه در رقم فلات و کمترین آن در رقم نیک­نژاد ثبت شد. آلودگی دو رقم فلات و مرودشت به قارچ بیمارگر منجر به کاهش تعداد سلول باکتری مستقر روی ریشه در مقایسه با شاهد شد. درحالی‌که در رقم نیک­نژاد، قارچ بیمارگر در کلونیزاسیون ریزوباکتری اثر افزایشی داشت. فعالیت آنزیم فنیل­­آلانین ­آمونیالیاز، به‌عنوان یکی از مهم­ترین آنزیم­های درگیر در واکنش­های دفاعی گیاه، در هر سه رقم گندم هنگامی‌که گیاه به‌طور همزمان به ریزوباکتری و قارچ بیمارگر آلوده شدند بیش از هنگامی بود که گیاهان به‌صورت جداگانه با هر یک از این عامل‌ها تیمار شدند. این موضوع، به‌احتمال بر نقش ریزوباکتری UTP100 در القای مقاومت سیستمیک از طریق افزایش فنیل­آلانین ­آمونیالیاز تأکید دلالت دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Interaction of Pseudomonas fluorescens UTP100, wheat and Fusarium culmorum pathogenic mushroom cultivars

نویسندگان [English]

  • Hoda Hosseinzadeh 1
  • Keyvan Behboodi 2
  • Jila Delkhah 3
1 Former M. Sc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Ph. D. Candidate, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

In this study, the effects of Pseudomonas fluorescens UTP100 as an rhizobacteria was investigated, on interaction of three wheat cultivars (Falat, Marvdasht, and Niknejad) whit Fusarium culmorum as an causal of agent wheat foot and root rot. Treatment of seeds by the rhizobacterium caused significant decrease in the infection percentage, and improved germination and growth parameters in all three studied cultivars. The colonization rate of the rhizobacteria was significantly different among wheat cultivars, and the highest and lowest numbers of bacterial cells/ mg root dry weight were estimated in Falat and Niknejad cultivars, respectively. Infection of Falat and Marvdasht cultivars by F. culmorum led to decreased rate of bacterial colonization, while the number of bacterial cells increased in Niknejad cultivar following infection by the pathogen. The activity of phenylalanine ammonia lyase (PAL) enzyme, as an important enzyme involved in plant defense against plant pathogens, increased significantly when wheat cultivars were infected simultaneously by both P. fluorescens and F. culmorum compared to when separately treated by each of these agents. This may imply that the rhizobacterium induce systemic resistance to the invaded plant through activation of PAL enzyme.

کلیدواژه‌ها [English]

  • Colonization
  • Fusarium culmorum
  • infection rate
  • phenylalanine ammonia lyase (PAL)
  • Pseudomonas fluorescens
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