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بررسی تأثیر غلظت‌های مختلف بوراکس بر میزان پراکسیداسیون لیپید و فعالیت سیستم آنتی‌اکسیدانی آنزیمی در موریانه Microcerotermes diversus (Isoptera: Termitidae)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد و دانشیار، دانشکدۀ کشاورزی، دانشگاه شهید چمران اهواز، ایران

2 دانشیار، دانشکدۀ کشاورزی، دانشگاه شهید چمران اهواز، ایران

چکیده

موریانۀ Microcerotermes diversus Silvesteri یکی از مهم‌ترین آفات محصولات چوبی و سلولزی در مناطق مختلف کشور از جمله استان خوزستان به شمار می‌آید. از سموم سازگار با محیط‌زیست که از طریق اختلال در فیزیولوژی و متابولیسم (سوخت‌وساز) حشرات تأثیر گذارند می‌توان به بورات‌ها از جمله بوراکس اشاره کرد. هدف از این بررسی تعیین نشانگرهای زیستی با استفاده از اندازه‌گیری فعالیت آنزیم‌های آنتی‌اکسیدانی (پاداکسندگی) و میزان پراکسیداسیون لیپید (چربی) در ایجاد تنش اکسیداتیو (اکسایشی) تحت تأثیر حشره‌کش است. بدین منظور تأثیر چهار غلظت 5/1 ، 2، 3 و 4 درصد بوراکس بر شاخص‌های مهم تنش اکسیداتیو از جمله مالون دی‌آلدهاید (MDA) و فعالیت آنزیم‌های آنتی‌اکسیدانی مانند سوپراکسید دیسموتاز (SOD)، کاتالاز (CAT)، پراکسیداز (POX)، آسکوربات پراکسیداز (APX) و گلوتاتیون ردوکتاز (GR) در طبقۀ کارگر بررسی شد. همۀ آزمایش‌ها در چهار تکرار انجام شد. نتایج حاصل نشان داد، با افزایش غلظت حشره‌کش، میزان غلظت MDA در همولنف نسبت به شاهد به‌صورت معناداری افزایش و فعالیت دو آنزیم GR و APX با افزایش غلظت کاهش می‌یابد، بیشترین و کمترین میزان فعالیت آنزیم SOD به ترتیب در تیمارهای 2 و 3 درصد، آنزیم CAT در تیمارهای 3 و 4 درصد و آنزیم POX در تیمارهای 4 و 3 درصد مشاهده شد. نتایج همچنین نشان داد، تیمار موریانه‌ها با غلظت‌های مختلف مورد نظر از بوراکس بر شاخص تنش مالون‌دی‌آلدهاید و فعالیت آنزیم‌های آنتی‌اکسیدانی POX، CAT، GR، APX و SOD تأثیرگذار است و می‌توان از آن‌ها به‌عنوان یک نشانگر زیستی برای تعیین تأثیر حشره‌کش در بدن موریانه استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of different concentrations of borax on lipid peroxidation and antioxidant enzyme system activities of Microcerotermes diversus (Isoptera: Termitidae)

نویسندگان [English]

  • Mina Darmiani 1
  • Behzad Habibpour 2
  • Payman Hassibi 2
1 M.Sc. Student and Associate Professor, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran
2 Associate Professor, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran
چکیده [English]

Microcerotermes diversus is an important pest of wooden and cellulosic products in diverse landscapes across Iran, including Khuzestan province. Borate toxicants (including borax) as environmentally sound pesticides affect insects by disrupting their physiology and metabolism. In this study, we measured activities of antioxidant enzymes and lipid peroxidation as biomarkers to determine the level of added oxidative stress in M. diversus caused by exposure to borax differentconcentrations. Then we measured the effects of four concentrations of borax, 1.5, 2, 3 and 4%, on oxidative stress biomarkers such as malondialdehyde (MDA) as a product of lipid peroxidation, and activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbat peroxidase (APX) and peroxidase (POX) on worker caste of termites. Each of trials was carried out in four replicates. Results showed that with increasing of borax concentration, the concentration of MDA significantly increased and GR and APX significantly decreased. The highest and lowest SOD activity compared with the control treatment occurred with the 2% and 3% borax treatments, respectively. The highest and lowest amount of CAT activity occurred in the 3% and 4%, and POX activity 4% and 3% borax treatments, respectively. Results showed that of the treatments termites’ with different concentrations of borax affected the stress biomarker including malondialdehyde, POX, CAT, GR, APX and SOD antioxidant enzyme levels. Therefore, antioxidant enzymes and MDA can be used as biomarkers to determine the effects of insecticides in the termite body.

کلیدواژه‌ها [English]

  • Biomarker
  • borates
  • oxidative stress
  • Malondialdehyde
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