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نقش بتا آمینوبوتریک اسید (BABA) در القای مقاومت علیه سفیدک داخلی خیار و فعالیت آنزیم‌های وابسته به دفاع

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه جیرفت

2 استادیار، دانشکدۀ کشاورزی، دانشگاه جیرفت

3 مربی، دانشکدۀ کشاورزی، دانشگاه جیرفت

چکیده

بیماری سفیدک داخلی خیار یکی از مهم‌ترین بیماری­های خیار در ایران و جهان است. در این پژوهش از ترکیب بتا آمینوبوتریک اسید (BABA) برای کاهش آسیب و زیان این بیماری به‌صورت غرقاب خاک و محلول‌پاشی برگ استفاده شد. برگ‌های گیاهان 24 ساعت پس از تیمار با BABA جدا و پس از مایه‌زنی با بیمارگر درون جعبه­های پلاستیکی با شرایط رطوبتی مطلوب بیمارگر قرار داده شدند. ارزیابی بیماری شش روز پس از تلقیح با بیمارگر انجام شد. نتایج نشان داد، کاربرد BABA به روش محلول‌پاشی برگ تأثیر معنی‌داری در کاهش بیماری نداشته و کاربرد خاک مصرف آن با 62 درصد کاهش بیماری همراه بوده و به لحاظ آماری با شاهد اختلاف معنی‌داری نشان داد. در مرحلۀ دوم آزمایش تأثیر این ترکیب در شرایط گلخانه علیه بیماری و تغییر در فعالیت آنزیم­های گلوکاناز، پلی فنل اکسیداز (PPO)، پراکسیداز (POX) و فنیل آلانین آمونیالیاز (PAL) در زمان‌های مختلف بررسی شد. نتایج نشان داد، فعالیت آنزیم­ها 48 ساعت پس از مایه­زنی با بیمارگر به‌سرعت افزایش داشته و پس از آن فعالیت آنزیم­های گلوکاناز، PAL و POX به‌تدریج کاهش یافت. درصورتی‌که فعالیت آنزیم PPO، پس از 48 ساعت افزایش، با سرعت بیشتری کاهش پیدا کرد. این موضوع بیانگر ایجاد حالت آماده‌باش در گیاه درنتیجۀ کاربرد این ترکیب است.

کلیدواژه‌ها


عنوان مقاله [English]

Role of β-amino butyric acid (BABA) in the induction of resistance against cucumber downy mildew and activities of defense-related enzymes

نویسندگان [English]

  • Samira mashayekhi 1
  • Hamidreza Alizadeh 2
  • Amirreza Amirmijani 2
  • Khadijeh Salari 3
1 Former M. Sc. Student, Faculty of Agriculture, University of Jiroft, Iran
2 Assistant Professor, Faculty of Agriculture, University of Jiroft, Iran
3 Instructor, Faculty of Agriculture, University of Jiroft, Iran
چکیده [English]

Cucumber downy mildew caused by Pseudoperonospora cubensis is one of the most important diseases of cucumberin Iran. In this investigation the role of β-Aminobutyric acid (BABA) in reducing this disease was studied via spraying of leaves and soil drench. The plants were treated with BABA and after 24 hours leaves were detached and inoculated with the pathogen and kept in a plastic box with favorable conditions for the disease agent. The disease was evaluated six days after inoculation with pathogen by scoring the leaves between 0-9 and the disease index calculated. Result showed that spraying the leaves by BABA had no effect on the disease reduction but soil application of this compound statistically reduced the disease (p≤0.05). The second phase of the experiment was done for evaluating the effect of BABA on the activity of enzymes such as β-1,4-Glucanase, phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase. Enzymes activity was assayed at 0, 24, 48, 72 and 96 hours after inoculation with the pathogen. Results showed an increas jn enzyme activity at 48-hours post-inoculation with the pathogen and then β-1,4-Glucanase, PAL, and POX enzymes activities decreased slowly, while PPO enzymes activity decreased rapidly.

کلیدواژه‌ها [English]

  • Phenylalanine ammonia-lyase (PAL)
  • Peroxidase (POX)
  • Polyphenol oxidase (PPO)
  • Priming
  • Pseudoperonospora cubensis
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