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آنالیز مولکولی جدایه‌های قارچ Fusarium culmorum عامل بیماری پوسیدگی طوقه و ریشۀ گندم با استفاده از نشانگر SSR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار بیماری‌شناسی گیاهی، گروه گیاهپزشکی، دانشگاه ایلام، ایران

2 دانشجوی سابق کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاهپزشکی، دانشگاه ایلام، ایران

چکیده

بیماری پوسیدگی طوقه و ریشۀ گندم برآمده از Fusarium culmorum یکی از مهم‌ترین بیماری‌های گندم در استان ایلام است. برای تعیین گوناگونی ژنتیکی بیمارگر در مزرعه‏های گندم استان ایلام، شمار 66 نمونۀ آلوده از مزرعه‏های شهرستان‌های گوناگون استان جمع‌آوری شد. پس از کشت، خالص‌سازی و شناسایی جدایه‌ها، آزمون مولکولی با به‌کارگیری پنج جفت آغازگر ریز ماهواره انجام شد. از آغازگرهای ریز ماهواره 25 آلل در جدایه‌ها تکثیر شد. میانگین تعداد آلل در هر جایگاه 2/5 مشاهده شد. شناسۀ چندشکلی نشانگرها در آغازگر F3 با 409/0 بیشترین و در آغازگر F11 با 179/0 کمترین مقدار را دارا بود. بر پایۀ خوشه‌بندی جدایه‏ها و با به‌کارگیری الگوریتم پیوست مجاور و نرخ همانندی جاکارد، در سطح همانندی 50 درصد جدایه‏ها در 18 گروه جدا از هم قرار گرفتند. نتیجه‏های تجزیه واریانس مولکولی نشان داد که 78 درصد از تنوع ژنتیکی در میان همۀ جدایه‌ها و تنها 22 درصد آن به منطقه‏های گوناگون جغرافیایی اختصاص دارد؛ بنابراین میان جدایه‌ها از منطقه‏های گوناگون همانندی ژنتیکی فراوانی وجود دارد. همانندی ژنتیکی بالا را می‌توان به مهاجرت ژن یا ژن‏مانه (ژنوتیپ) در اثر عامل‏های گوناگون نسبت داد.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular analysis of Fusarium culmorum isolates caused by crown and root rot of wheat by the SSR marker

نویسندگان [English]

  • Khoshnood Nourollahi 1
  • Farhad Gholami 2
1 Associate Professor, Department of Plant Protection, Ilam University, Iran
2 Former M. Sc. Student, Department of Plant Protection, Ilam University, Iran
چکیده [English]

Crown and root rot caused by Fusarium culmorum is one of the most important wheat diseases in Ilam. In order to determine genetic diversity, 66 samples were collected from wheat farms in different regions of Ilam province. The molecular test was carried out with a set of five pairs of SSR primers after purification and identification of the isolates. The SSR primers amplified a total of 25 alleles. The average allele number was 5.2 per each primer. The polymorphism index content value was the highest in primers F3 with 0.409 and the lowest in primers F11 with 0.179. Cluster analysis using the Neighbor-Joining method and Jaccard's coefficient in 50% similarity level divided the isolates into 18 groups. Results of AMOVA showed 78% of the genetic diversity related to isolates and 22% related to different geographical regions. Therefore, there is a high genetic similarity between isolates from different geographic regions. High genetic similarity can be attributed to the emigration of gene or genotype as a result of various factors.

کلیدواژه‌ها [English]

  • Fusarium culmorum
  • Genetic diversity
  • SSR
  • Wheat
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