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آزمون نتاج جدایۀ ایرانی ویروئید اگزوکورتیس مرکبات در دو پایۀ حساس و متحمل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه گیاه‌پزشکی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 دانشیار، گروه گیاه‌پزشکی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

4 استاد، گروه گیاه‌پزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

چکیده

ویروئید اگزوکورتیس مرکبات Citrus exocortis viroid (CEVd)، عامل یکی از بیماری‌های‏ مهم اقتصادی به نام اگزوکورتیس مرکبات است‏. در این پژوهش، چندشکلی توالی نوکلئوتیدی و ساختار جمعیت CEVd در دو میزبان متحمل و حساس (به ترتیب، نارنج (Citrus aurantium L.) و سیترنج ((Poncirus trifoliata (L.) Raf. × C. sinensis (L.)) بررسی شد. رونوشت تمام طول برآمده از جدایۀ ایرانی CEVd-S1 در شرایط درون شیشه‌ای‏ جهت آلوده‌سازی‏ مکانیکی نهال‌ها‏ استفاده شد. تنوع ژنتیکی جمعیت‌های‏ CEVd با به‌کارگیری چندشکلی فضایی تک‌رشته‌ای‏ (SSCP) و توالی‌یابی‏ نوکلئوتیدی تعیین شد. بررسی ژنوم ویروئید همسانه‏سازی‏شده در این دو میزبان، تأثیر گونۀ میزبان بر تغییرهای ژنتیکی جمعیت برآمده از یک جدایۀ CEVd را آشکار کرد. نتیجه‏های این پژوهش نشان دادند که ساختار جمعیتی ویروئید اگزوکورتیس مرکبات در دو میزبان حساس و متحمل متفاوت و در میزبان متحمل متنوع‏تر از میزبان حساس است. بررسی ساختار ثانویۀ ترمودینامیکی واریانت‌ها‏ نشان داد که تغییرهای شناسایی‌شده در ژنوم ویروئید در این پژوهش تأثیر چشمگیری در ساختار کلی میله‌ای ‏‏شکل ویروئید نداشته است.

کلیدواژه‌ها


عنوان مقاله [English]

Progeny analysis of Iranian Citrus exocortis viroid isolate in two sensitive and tolerant rootstocks

نویسندگان [English]

  • Marzie Ghobakhloo 1
  • Akbar Dizadji 2
  • Ahad Yamchi 3
  • Heshmat Rahimian 4
1 Ph.D. Student, Department of Plant Protection, Faculty of Agricultural Sciences & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Plant Protection, Faculty of Agricultural Sciences & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Department of Plant breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources. Gorgan, Iran
4 Professor, Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

Citrus exocortis viroid (CEVd) is the causal agent of exocortis disease, the economically important viroid disease of citrus. In this research, sequence polymorphism and population structure of CEVd were investigated in CEVd tolerant and sensitive hosts (sour orange (Citrus aurantium L.) and citrange (Poncirus trifoliata (L.) Raf. × C. sinensis (L.), respectively) seedlings. Full-length in vitro transcript of single sequence CEVd-S1 isolate was used for mechanical inoculation. The genetic diversity of CEVd populations was estimated in two citrus hosts by single-strand conformation polymorphism (SSCP) and sequencing. The analysis of cloned DNAs recovered from infected hosts by this isolate demonstrated that host species was effective on the variability within a single CEVd isolate. The amount and composition of the genetic diversity were different among the two hosts, and was higher in the tolerant host compared with the sensitive one. Furthermore, the analysis of thermodynamic secondary structures illustrated that nucleotide changes identified in this study did not induce major modifications in the viroid rod-like secondary structure.

کلیدواژه‌ها [English]

  • Citrus exocortis viroid
  • Population structure
  • SSCP
  • Variant
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