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ارزیابی دگرگونی‌های زیست‏ شیمیایی و مولکولی گیاه گوجه‌فرنگی در برهمکنش با بیمارگر Alternaria solani

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی بیماری‏شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی دانشگاه زابل، ایران

2 دانشیار، گروه گیاه‌پزشکی، دانشکده کشاورزی دانشگاه زابل، ایران

3 استادیار، گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم، تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران

4 استادیار، گروه بیولوژی، دانشگاه یزد، یزد، ایران

چکیده

لکه موجی از بیماری‌های مهم گیاه گوجه‌فرنگی است که سبب کاهش کمی و کیفی محصول می‌شود. با توجه به کاربرد بی‌رویۀ آفت‏کش‏ها در مهار این بیماری شناسایی سازوکارهای دفاعی گیاه در برابر بیمارگر می‌تواند در شناساندن ارقام مقاوم و مهار زیان بیمارگر سودمند واقع شود. در این پژوهش برخی دگرگونی‌های زیست‏شیمیایی و الگوی تظاهر ژن‌های PR1b1 و WRKY33 به روش qRT-PCR در رقم مقاوم Super 2270 و حساس CH Flat در برابر Alternaria solani  بررسی شدند. نمونه‌برداری در پنج بازۀ زمانی با سه تکرار انجام شد. نتیجه‏ها نشان دادند که فعالیت گوایکول پراکسیداز، کاتالاز و سوپر اکسید دیسموتاز و میزان رونوشت ژن‌های موردبررسی پس از مایه‌زنی گیاه با بیمارگر در رقم مقاوم و حساس افزایش یافت ولی میزان این افزایش در رقم مقاوم بیشتر بود. میزان تجمع پراکسید هیدروژن در رقم مقاوم در ساعت‏های نخستین به‌سرعت افزایش یافت و به بیشترین مقدار خود رسید درحالی‌که تجمع آن در رقم حساس در مراحل پسین آلودگی نیرومندتر و بیشتر بود. نتیجه‏ها نشان دادند که القاء پاسخ‌های اکسیدانی و آنتی‌اکسیدانی و هم‌چنین فعالیت ژن‌های موردبررسی بخشی از سازوکارهای دفاعی گوجه‌فرنگی در برابرA. solani است.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of biochemical and molecular changes of tomato plants interacted with Alternaria solani

نویسندگان [English]

  • Batul Sadeghi 1
  • Mohammad Salari 2
  • Saeid Mirzaei 3
  • Naser Panjehkeh 2
  • Seyed Kazem Sabbagh 4
1 Ph. D. Candidate, Department of Plant Protection, University of Zabol, Iran
2 Associate Professor, Department of Plant Protection, University of Zabol, Iran
3 Assistant Professor, Department of Biotechnology, Institute of Science, High technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
4 Associate Professor, Department of Biology, Faculty of Science, Yazd University, Iran
چکیده [English]

Early blight disease is one of the most common foliar diseases of tomato, which causes a great reduction in the quantity and quality of its yield. Regarding the use of fungicides in controlling this disease, identification of plant defense mechanism against the pathogen can be useful in introducing resistant cultivars and controlling the damage of the pathogen. In this research, several biochemical changes and expression patterns of PR1b1 and WRKY33 genes were investigated by qRT-PCR in a resistant cultivar Super 2270 and a susceptible cultivar CH Flat infected with the fungus Alternaria solani. Sampling was done during five intervals with three replications. The hydrogen peroxide accumulation, activity of defense enzymes Guacul peroxidase, Catalase and Superoxide dismutase enzymes, and the number of transcripts of genes was found to be increased in a challenge with the pathogen. The activity of these enzymes and genes were higher in the resistant cultivar. H2O2 accumulated rapidly in the resistant cultivar leaf tissues and peaked during the early stages of infection, whereas accumulation was stronger and more intense in the susceptible cultivar tissues in later stages.These results indicated that the induction of oxidant/antioxidant responses and the activity of genes in this study are a part of the tomato defense mechanism against the necrotrophic fungus A.solani.

کلیدواژه‌ها [English]

  • Biochemical
  • defense reaction
  • gene expression
  • pathogenecity
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