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مطالعه بیماری شانکر سطحی پوست گردو در استان کرمان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان

2 دانشیار، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان

3 استادیار، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان

چکیده

بیماری شانکر پوستی گردوی ایرانی (Juglans regia L.) با عامل Brenneria nigrifluens یکی از بیماری‌های مهم درختان گردو است. تشخیص به‌موقع عامل بیماری، نقش مهمی در جلوگیری از خسارت‌های جبران‌ناپذیر آن دارد. در پژوهش حاضر، 57 جدایه از درختان دارای علائم بیماری، از مناطق مختلف استان کرمان جمع‌آوری شدند. جدایه‌ها بر اساس ویژگی‌های فنوتیپی و بیوشیمیایی به دو گروه تقسیم شدند. با استفاده از آغازگرهای اختصاصی و تکثیر قطعه‌ای از DNA به طول 255 جفت باز، جدایه‌های متعلق به گونۀ B. nigrifluens از عوامل همراه با عامل بیماری، تفکیک شدند. بیماری‌زایی جدایه‌ها بر روی میوه‌های نارس گردو ارزیابی شد. جهت شناسایی مولکولی برخی جدایه‌ها، بخشی از اپران ریبوزومی نیز در آن‌ها تکثیر و توالی‌یابی شد. نتایج نشان دادند که باکتری B. nigrifluens با تنوع ژنتیکی قابل‌ملاحظه، در مناطق مختلف گردو کاری استان کرمان گسترش داشته و افزون بر آن، عوامل دیگری از جنس Gibbsiella نیز می‌توانند در ایجاد یا تشدید علائم بیماری دخالت داشته باشند که نیازمند پژوهش‌های دیگری است.

کلیدواژه‌ها


عنوان مقاله [English]

Study on shallow bark canker disease of walnut in Kerman province

نویسندگان [English]

  • Elahe Sadeghi 1
  • Pejman Khodaygan 2
  • Ahmad Hosseini 3
  • Roohallah Saberi-Riseh 2
1 M. Sc. Student, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran
2 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran
3 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran
چکیده [English]

Walnut is one of the important nuts with a high economic value. Shallow bark canker, caused by Brenneria nigrifluens, is one of the most dangerous diseases of the walnut trees. Early detection of the causal agent has an important role in preventing economic and irreparable losses caused by this disease. In this study, 57 bacterial strains were isolated from walnut trees with the typical lesions of the shallow bark cankers from different regions of Kerman province. These isolates were divided into two groups, based on phenotypic and biochemical characteristics and then, amplification product of the expected size (255 bp) was obtained with genomic DNA using specific pair primers in B. nigrifluens isolates.Pathogenicities of the isolates were assessed on immature walnut fruits. To identify the causal and the associated bacteria molecularly, a part of 16s rDNA gene sequence was analyzed. Results showed that B. nigrifluens with a significant genetic diversity spread over different regions of Kerman province. In addition, the genus Gibbsiella can probably contribute to the increase of the disease severity, which requires further studies.

کلیدواژه‌ها [English]

  • Brenneria
  • Kerman province
  • Shallow bark canker of walnut
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