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تأثیر آلودگی ویروس موزاییک خیار و ویروس موزاییک کلم‌گل بر مقاومت کلزا به ویروس موزاییک شلغم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس

2 دانشیار، گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس

چکیده

ویروس موزاییک شلغم (Turnip mosaic virus, TuMV) از مزارع کلزا به‌طور گسترده گزارش شده است. کشت رقم­های مقاوم بهترین راه کنترل بیماری‌های ویروسی است. بااین‌وجود گیاهان مقاوم به یک ویروس در آلودگی هم‌زمان با سایر ویروس‌ها ممکن است مقاومت خود را از دست بدهند. ازاین‌رو در پژوهش حاضر تأثیر آلودگی مخلوط TuMV، با ویروس موزاییک خیار (Cucumber mosaic virus, CMV) و ویروس موزاییک کلم‌گل (Cauliflower mosaic virus, CaMV) روی مقاومت یک رقم متحمل کلزا (کرج 3) و یک لاین کلزای تراریخته از رقم بهارۀ RGS003 دارای سازۀ سنجاق سری با مقاومت بالا در برابرTuMV در شرایط گلخانه بررسی شد. مایه‌زنی گیاهان به روش مکانیکی و ارزیابی غلظت نسبی TuMV با استفاده از روش RT-PCR نیمه­کمی انجام گرفت. نتایج این پژوهش نشان دادند آلودگی هم‌زمان TuMV و CaMV تأثیری روی مقاومت کلزای تراریخته مقاوم به TuMV ندارد. نتایج RT-PCR نیمه کمی نیز اختلاف معنی‌داری در غلظت TuMV در حضور CaMV نشان ندادند. درحالی‌که برهمکنش هم‌افزایی بین TuMV و CMV قادر به غلبه بر مقاومت گیاهان کلزای تراریخته در برابر TuMV شد. نتایج RT-PCR نیمه کمی نیز نشان دادند غلظت TuMV در گیاه تراریخته در حضور CMV افزایش یافت، درحالی‌که در رقم کرج 3 غلظت TuMV تحت تأثیرCMV قرار نگرفت. بنابراین برای معرفی گیاهان تراریخته مقاوم به TuMV لازم است سازه‌ای طراحی شود که گیاه تراریخته هم‌زمان به TuMV و CMV مقاوم شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Cucumber mosaic virus and Cauliflower mosaic virus Infections on the Resistance of Canola to Turnip mosaic virus

نویسندگان [English]

  • Seyedeh Fatemeh Nourbakhsh Shourabi 1
  • Masoud Shams-bakhsh 2
1 Former M. Sc. Student, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Turnip mosaic virus (TuMV) has frequently been reported from canola fields. Cultivation of resistant varieties is the best method to control viral diseases. However, plants that are resistant to a virus may lose their resistance in co-infection with other viruses. Therefore, in the present study the effect of mixed infection of TuMV with Cucumber mosaic virus (CMV) and Cauliflower mosaic virus (CaMV) on the resistance of a tolerant canola cultivar (Karaj 3) and a transgenic canola of RGS003spring variety with hairpin construct and high resistance to TuMV was investigated under greenhouse conditions. Canola plants were inoculated mechanically and then, relative TuMV content was assayed by semi-quantitative RT-PCR. Results demonstrated that mixed infection of TuMV and CaMV did not affect the resistance of transgenic canola plants against TuMV. Also, there was not any significant difference in TuMV content in the presence of CaMV. However, synergistic interaction between TuMV and CMV could overcome the resistance of canola plants to TuMV. The result of semi-quantitative RT-PCR indicated that TuMV accumulation was enhanced in the presence of CMV in transgenic plants, whereas TuMV levels were not affected by co-infection with CMV in Karaj 3. Therefore, it is concluded that for the introduction of TuMV-resistant transgenic plants, it is necessary to design a construct that the resulting transgenic plants would also be resistant to CMVsimultaneously.

کلیدواژه‌ها [English]

  • Brassica napus
  • RNA silencing suppression
  • virus interactions
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