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معرفی، بررسی مکانیسم‌های عمل و توصیف ژنومی باکتری پروبیوتیک گیاهی ‏Bacillus velezensis

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 دانشیار، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه

4 دانشیار، گروه میکروبیولوژی، دانشگاه کرنل، ایالت نیویورک

چکیده

باکتری ‌Bacillus velezensis یکی از عوامل پروبیوتیک گیاهی می‌باشد که اخیراً به‌عنوان یکی از ابزار‌های قوی بیوکنترل مورد توجه قرار گرفته است. هدف از این پژوهش معرفی و بررسی برخی از خصوصیات باکتری B. velezensis می‌باشد که از ریزوسفر گوجه‌فرنگی شهرستان کرمانشاه جداسازی شد. در این پژوهش باکتری B. velezensis به­ترتیب موجب افزایش 86/78، 87/56 و 83/45 درصدی طول شاخساره، طول ریشه و وزن خشک گیاه گوجه‌فرنگی در گلخانه و درصد جوانه‌زنی و شاخص بنیه بذر در شرایط آزمایشگاهی گردید. علاوه بر این، برخی از خصوصیات پروبیوتیکی این جدایه از جمله تولید سیدورفور، سیانید هیدروژن و اندول استیک اسید در آزمایشگاه مورد ارزیابی قرار گرفتند. اثر بازدارندگی این سویه روی قارچ Fusarium oxysporum f. sp. lycopersici به روش کشت متقابل و ترکیبات فرّار ضدقارچی به‌ترتیب به میزان 83 و 58 درصد بود. در بررسی‌های گلخانه‌ای نیز باعث کاهش بروز 5/87 درصدی علائم بیماری گردید. در نهایت ژنوم کامل باکتری با استفاده از تکنولوژی ایلومینا تعیین توالی گردید. سپس در پایگاه RAST مکان‌های ژنومی و توالی‌های رمزگردان مشخص گردیده و در پایگاه NCBI با توالی‌های موجود مقایسه شد. نتایج نشان داد که این جدایه با احتمال 100 درصد متعلق به گونه B. velezensis است. اندازه ژنوم این جدایه 4132868 جفت باز و شامل 4329 توالی رمزگردان می‌باشد. این تحقیق اثباتی بر معرفی گونه B. velezensis به­عنوان باکتری پروبیوتیک گیاهی است.

کلیدواژه‌ها


عنوان مقاله [English]

Introduction, mechanisms of action and genomic description in plant probiotic ‎bacterium Bacillus velezensis

نویسندگان [English]

  • Sonia Seifi 1
  • Keyvan Behbodi 2
  • Rouhallah Sharifi 3
  • James. P Shapleigh 4
1 Ph. D. Candidate, Department of Plant Protection, College of Agirculture & Natural Resources, University ‎of Tehran, Karaj, Iran
2 Associate Professor, Department of Plant Protection, College of Agirculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Faculty of Agriculture, Razi University, Kermanshah, Iran
4 Associate Professor, Department of Microbiology, Cornell University, Ithaca, New York, USA
چکیده [English]

Bacillus velezensis is one of the plant probiotic bacteria that has been recently considered as one of the most powerful biocontrol agents. The purpose of this study is to introduce and investigate some of the characteristics of B. velezensis bacterium, which has been isolated from tomato rhizosphere in Kermanshah Province. In this study, B. velezensis increased shoot length, root length and dry weight of tomato plants by 78.86%, 56.68%, and 45.83%, respectively in comparison to uninoculated control; it also promoted seed germination percentage and seed vigor index under in vitro condition. Furthermore, some of the biocontrol properties of this strain such as the production of siderophore, HCN, and indole-3-acetic acid and solubilization of phosphate were evaluated. Biocontrol bioassay of this strain against Fusarium oxysporum f.sp. lycopersici under in vitro condition revealed that this strain can inhibit the mycelial growth in both dual culture and volatile compounds methods about 83% and 58%, respectively. The pot experiment results showed that B. velezensis caused a reduction in disease symptoms up to 87%. Finally, the whole genome sequence of this strain was identified using Illumina HiSeq2500 technology. Then genomic locations and coding sequences were identified in RAST database. In addition, the sequencing data were compared with existing sequences in the NCBI database. Results showed that this isolate has high similarity to (%100) B. velezensis. The genome of this isolate contains 4132868bp and 4329 CDs. The results suggest that B. velezensis has a good potential to be introduced as a plant probiotic bacterium.

کلیدواژه‌ها [English]

  • biological control
  • Bacillus velezensis
  • Tomato fusarium wilt disease
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