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شناسایی مولکولی باکتری‌های اندوفیت‌ مرکبات در شرق استان گیلان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده تولید‌گیاهی، دانشگاه علوم‌کشاورزی و منابع‌طبیعی گرگان، گرگان، ایران.

2 دانشکده تولید گیاهی گروه گیاه پزشکی دانشگاه علوم کشاورزی منابع طبیعی گرگان

3 ریس پژوهشکده مرکبات و میوه های نیمه گرمسیری

4 گروه گیاهپزشکی- دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

این تحقیق به منظور شناسایی اندوفیت‌های باکتریایی درخت‌‌های مرکبات بالای پنج سال در شرق استان گیلان انجام شد. تعداد 63 باکتری اندوفیت‌ از برگ‌های سالم نارنج، پرتقال محلی و تامسون ناول، نارنگی‌ انشو، لیموشیرین، کامکوات و بالنگ جداسازی گردید. جدایه‌های بدست آمده براساس شکل و رنگ‌ پرگنه گروه‌بندی شدند، سپس خصوصیات ریخت-شناسی و بیوشیمیایی شش جدایه از آن‌ها به عنوان نماینده گروه‌های مختلف مورد ارزیابی قرار گرفت. براساس ارزیابی-های مولکولی و مطالعات تبارزایی ناحیه 16S rRNA مشخص گردید که این جدایه‌ها به گونه‌های Bacillus megaterium،Bacillus cereus، ‌Arthrobacter agilis، Acinetobacter junii و Pseudomonas azotoformans تعلق دارند. که از این میان سه گونه‌ A. agilis، A. junii و P. azotoformans برای اولین بار به عنوان باکتری‌های اندوفیت مرکبات از ایران و جهان گزارش می‌گردند. ویژگی‌های ریخت‌شناسی، بیوشیمیایی‌، مولکولی و تجزیه و تحلیل‌های تبارزایی به خوبی قادر به تمایز جنس‌ها و گونه‌ها‌ی اندوفیت جدا شده می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular identification of citrus endophytic bacteria in the east of guilan provience

نویسندگان [English]

  • S.leila Akbari kiarood 1
  • kamran Rahnama 2
  • morteza Golmohammadi 3
  • Saeed Nasrollanejad 4
1 Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Faculty member
3 Assistant Professor Horticultural Science Research Institute, Citrus and Subtropical Fruits Research Center
4 Department of Plant Protection, Gorgon University of Agricultural Sciences & Natural Resources
چکیده [English]

This research was conducted to identify endophytic bacteria over five-year old citrus trees in the east of Guilan province. A total of 63 endophytic bacteria were isolated from symptomless leaves of Citrus aurantium, Citrus sinensis, Citrus sinensis var. thomson, Citrus unshiu, Citrus limon, Fortunella margarita and Citrus medica. The isolates were grouped based on colony type and color. Then the morphological and biochemical characteristics of the six isolates were assessed as candidate of different groups. According to the molecular studies and phylogenetic analyses of 16srRNA region these isolates were identified as Bacillus megaterium, Bacillus cereus, Arthrobacter agilis, Acinetobacter junii and Pseudomonas azotoformans species. . Among them, three species including A. agilis, A. junii and P. azotoformans are reported as citrus endophytic bacteria from Iran and the world for the first time. The morphological, biochemical, molecular and phylogenetical analyses are able to differentiate genus and species of the endophyte isolates.

کلیدواژه‌ها [English]

  • Bacterial endophyte
  • citrus
  • 16SrRNA
  • phylogeny
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