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ارزیابی مقاومت و واکنش آنزیمی ارقام مختلف باقلا در برابر لکه برگی آلترناریایی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 گروه گیاه پزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 گروه باغبانی و گیاهپزشکی، دانشکده کشاورزی، دانشگاه صنعتی شاهرود

چکیده

باقلا (Vicia faba) یکی ازمهم‌ترین لگوم‌های دانه‌ای در گیاهان زراعی می‌باشد. بیماری لکه‌برگی آلترناریایی یکی از بیماری‌های باقلا در استان گلستان و مازندران و عامل خسارت و کاهش عملکرد در این گیاه می‌باشد. در تحقیق حاضر از مزارع باقلا آلوده به بیماری در استان‌های گلستان و مازندران نمونه‌برداری انجام شد. جدایه بدست آمده براساس خصوصیات ریخت‌شناسی و مولکولی Alternaria alternata شناسایی شد. به منظور ارزیابی مقاومت نسبی نه رقم باقلا نسبت به این بیماری در شرایط گلخانه، سطح زیر منحنی پیشرفت بیماری (AUDPC) اندازه‌گیری شد. همچنین تغییرات برخی از آنزیم‌های مرتبط با مقاومت در رقم حساس و مقاوم در زمان‌های مختلف پس از آلودگی بررسی شد. نتایج نشان داد اختلاف معنی‌داری در سطح 5 درصد در نرخ حساسیت به بیماری در ارقام مختلف در مقابل A. alternata وجود دارد. رقم اوتونو با بیشترین مقدار AUDPC حساس‌ترین و ارقام زرشکی و زینامورا با کمترین مقدار، مقاوم‌ترین ارقام بودند. همچنین نتایج داده‌های فعالیت آنزیم‌های آنتی‌اکسیدان نشان‌دهنده اختلاف معنی‌دار در سطح یک درصد در فعالیت هر سه آنزیم پراکسیداز، آسکوربات پراکسیداز و کاتالاز پس از وقوع بیماری می‌باشد. افزایش در مقادیر آنزیم‌ها در تعامل ناسازگار (رقم مقاوم زرشکی) در مقایسه با تعامل سازگار (رقم حساس اوتونو) بیشتر بود که نقش این آنزیم‌ها در فعال‌سازی واکنش‌های دفاعی گیاه باقلا در تعامل با بیمارگر و بروز مقاومت را نشان می‌دهد. به طور کلی نتایج نشان داد که رقم زرشکی با مقدار کم AUDPC و بیان بالای آنزیم‌های آنتی اکسیدانی از پتانسیل بالایی برای اصلاح ژنتیک در مقاومت به بیماری لکه‌برگی آلترناریایی برخوردار می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of resistance and enzyme production of faba bean cultivars against Alternaria leaf spot

نویسندگان [English]

  • Mohammad Ali Tajick Ghanbary 1
  • Soghra Taherpour Kolaei 2
  • Valiollah Babaeizad 1
  • Shideh Mojerlou 3
1 Department of Plant Protection, Faculty of Agronomic sciences, Sari Agriculture and Natural Resources University
2 Department of Plant Protection, Faculty of Agronomic sciences, Sari Agriculture and Natural Resources University
3 Department of Horticulture and Plant Protection, Faculty of Agriculture, Shahrood University of Technology
چکیده [English]

Faba Bean (Vicia faba) is an important legume among cultivated plants and Alternaria leaf spot is a serious disease that causes broad damages and yield loss in Golestan and Mazandaran provinces. In current research, sampling was carried out from Mazandaran and Golestan provinces. Morphological and molecular surveys of the obtained isolates showed that they belong to A. alternata species. In order to evaluate resistance of nine Faba Bean cultivars against Alternaria leaf spot, area under disease progress curve (AUDPC) were calculated in greenhouse condition. In addition, variation of some resistance related enzymes between resistant and susceptible lines were tested at different time intervals after inoculation. Statistical analysis showed significant difference between cultivars (P≤ 0.05). The Otono cultivar with the highest AUDPC and Zereshki cultivar with the least AUDPC were suggested as susceptible and resistance cultivars, respectively. The ANOVA of antioxidant enzymes showed positive effects on CAT, APX and GPX levels after inoculation (P≤ 0.01). Increasing in the amount of enzymes in incompatible interaction (Zereshki resistant cultivar) was greater than that of compatible interaction (Otono susceptible cultivar), indicates the role of these enzymes in activating plant defense responses in Faba Bean to interact with the pathogen and induce resistance. In general, the results showed that Zereshki cultivar with low level of AUDPC and high expression of antioxidant enzymes has high potential to be used in breeding programs for resistance to Alternaria leaf spot.

کلیدواژه‌ها [English]

  • Catalase
  • Ascorbate peroxidase
  • Area Under Disease Progress Curve
  • Alternaria leaf spot
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