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بهینه‌سازی اثرات ضد قارچی اسانس‌های دارچین، آویشن شیرازی، و مرزه خوزستانی علیه قارچ کپک آبی (Penicillium expansum) با استفاده از روش سطح پاسخ

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری مکانیک بیوسیستم، گروه مهندسی مکانیک بیوسیستم، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، ایران

2 گروه مهندسی بیوسیستم، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 دانشیار گروه مهندسی شیمی، دانشکده فنی و مهندسی، دانشگاه محقق اردبیلی، ایران

4 دانشیار گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، ایران

5 دانشیار گروه گیاه پزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، ایران

چکیده

استفاده از اسانس‌ها به‌عنوان عوامل ضد قارچی طبیعی و جایگزین‌هایی برای قارچ‌کش‌های شیمیایی توجه زیادی را به خود جلب کرده است. در این تحقیق، ترکیبات شیمیایی و خواص ضد قارچی سه اسانس دارچین، آویشن شیرازی و مرزه خوزستانی با هدف بررسی اثر غلظت اسانس و زمان تماس در کنترل قارچ عامل کپک آبی (Penicillium expansum) و مدل‌سازی و بهینه‌یابی خواص ضد قارچی اسانس‌های موردنظر با استفاده از روش سطح پاسخ بررسی شده است. بر اساس نتایج حاصل از GC-MS، ترکیبات اصلی برای اسانس دارچین، سینامالدهاید (۸۲/۸۰ درصد)، برای آویشن شیرازی ترکیبات تیمول (۶۸/۳۲ درصد)، کارواکرول (۵۷/۳۰ درصد) و پاراسیمن (۹۴/۸ درصد) و برای مرزه خوزستانی، کارواکرول (۴۳/۳۸ درصد)، گاماترپینن (۸۹/۲۱ درصد)، پاراسیمن (۵۵/۱۶ درصد) و آلفاترپینن (۷۶/۵ درصد) تعیین شد. نتایج به دست آمده از کشت برون تنی قارچ در محیط‌های حاوی اسانس نشان داد که در هر سه اسانس با افزایش غلظت، شاخص خاصیت ضد قارچی نیز افزایش می‌یابد و اثر زمان در هر سه اسانس مشابه بوده و با گذشت زمان، این شاخص کاهش می-یابد. به‌علاوه، از نتایج بهینه‌یابی مشخص شد که اسانس دارچین و مرزه خوزستانی به ترتیب در غلظت ۶۳۳/۲۸۸ و μL.L-1 ۸۴۱/۵۹۰ و زمان ۶۰۷/۱۰۸ و h ۵۴۹/۲۳۸، بیش‌ترین و کم‌ترین خاصیت مهارکنندگی رشد قارچ P. expansum را دارند. علاوه بر این، نتایج این تحقیق نشان داد که روش سطح پاسخ می‌تواند به‌عنوان روشی مناسب و دقیق برای مدل‌سازی و بهینه‌یابی فعالیت ضد قارچی این اسانس‌ها به کار رود.

کلیدواژه‌ها


عنوان مقاله [English]

Optimizing the antifungal effects of Cinnamomum zeylanicum, Zataria multiflora, and Satureja khuzestanica essential oils against the blue mold fungus (Penicillium expansum) using Response Surface Methodology

نویسندگان [English]

  • Mahsa Sadat Razavi 1
  • Abdollah Golmohammadi 2
  • Ali Nematollahzadeh 3
  • Alireza Ghanbari 4
  • Mahdi Davari 5
1 PhD student, Department of Biosystems Engineering, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Biosystems Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 3Associated Professor, Department of Chemical Engineering, University of Mohaghegh Ardabili, Ardabil, Iran
4 Associated Professor, Department of Horticultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
5 5Associated Professor, Department of Plant Protection, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Utilizing the essential oils (EOs) as natural antifungal agents and alternatives for chemical fungicides has increased the attentions. In this study, chemical components and antifungal activity of Cinnamomum zeylanicum (CEO), Zataria multiflora (ZEO), and Satureja khuzestanica (SEO) plants essential oil (EO) were identified to assess their effect in term of concentration and exposure time on control of Penicillium expansum and also, modeling and optimizing antifungal properties of these EOs via response surface methodology (RSM). The main components of three EOs detected by GC-MS analysis were as follows: Cinnamaldehyde (80.82%) for CEO, Thymol (32.68%), Carvacrol (30.57%), p-Cymene (8.94), and γ-Terpinene (5.96%) for ZEO and Carvacrol (38.43%), γ-Terpinene (21.89%), p-Cymene (16.55%), and α-Terpinene (5.76%) for SEO. Antifungal index (AI) in all EOs increased against the increment of concentration but the effect of time was vice versa; with passage of time, the AI decreased in all EOs. Moreover, based on the optimization results, it was determined that CEO and SEO at concentration of 288.633 and 590.841 μL.L-1 and time of 108.607 and 238.549 h had the highest and lowest inhibitory effect, respectively, on growth of Penicillium expansum. Furthermore, the results of this study showed that the response surface method can be applied to model and optimize the antifungal activity of these EOs as a suitable and accurate method.

کلیدواژه‌ها [English]

  • Essential oil
  • Inhibition
  • Optimization
  • response surface methodology
  • Penicillium expansum
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