نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری مکانیک بیوسیستم، گروه مهندسی مکانیک بیوسیستم، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، ایران
2 گروه مهندسی بیوسیستم، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران
3 دانشیار گروه مهندسی شیمی، دانشکده فنی و مهندسی، دانشگاه محقق اردبیلی، ایران
4 دانشیار گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، ایران
5 دانشیار گروه گیاه پزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Utilizing the essential oils (EOs) as natural antifungal agents and alternatives for chemical fungicides has increased the attentions. In this study, chemical components and antifungal activity of Cinnamomum zeylanicum (CEO), Zataria multiflora (ZEO), and Satureja khuzestanica (SEO) plants essential oil (EO) were identified to assess their effect in term of concentration and exposure time on control of Penicillium expansum and also, modeling and optimizing antifungal properties of these EOs via response surface methodology (RSM). The main components of three EOs detected by GC-MS analysis were as follows: Cinnamaldehyde (80.82%) for CEO, Thymol (32.68%), Carvacrol (30.57%), p-Cymene (8.94), and γ-Terpinene (5.96%) for ZEO and Carvacrol (38.43%), γ-Terpinene (21.89%), p-Cymene (16.55%), and α-Terpinene (5.76%) for SEO. Antifungal index (AI) in all EOs increased against the increment of concentration but the effect of time was vice versa; with passage of time, the AI decreased in all EOs. Moreover, based on the optimization results, it was determined that CEO and SEO at concentration of 288.633 and 590.841 μL.L-1 and time of 108.607 and 238.549 h had the highest and lowest inhibitory effect, respectively, on growth of Penicillium expansum. Furthermore, the results of this study showed that the response surface method can be applied to model and optimize the antifungal activity of these EOs as a suitable and accurate method.
کلیدواژه‌ها [English]
10. Bus, V., Bongers, A., & Risse, L. (1991). Occurrence of Penicillium digitatum and P. italicum resistant to benomyl, thiabendazole and imazalil on citrus fruit from different geographic origins. Plant Disease, 75(11), 1098-1100.
11. Davari, M., & Ezazi, R. (2017). Chemical composition and antifungal activity of the essential oil of Zhumeria majdae, Heracleum persicum and Eucalyptus sp. against some important phytopathogenic fungi. Journal de mycologie medicale, 27(4), 463-468.
12. Ebadollahi, A., Taghinezhad, E., & Davari, M. (2018). Optimization of antifungal and insecticidal effects of garden thyme (Thymus vulgaris) essential oil through Response Surface Methodology. Journal of biological control of pests and plant diseases, 7(1), 9-19. (In Farsi)
13. Eren, İ., & Kaymak-Ertekin, F. (2007). Optimization of osmotic dehydration of potato using response surface methodology. Journal of Food Engineering, 79(1), 344-352.
14. Etemadi, N. A., Behdad, M., & Zeinali, H. (2012). Antifungal effects of three plant essential oils against Botrytis cinerea: the cause of gray mold on strawberry. Journal of Research in Agricultural Science, 8(2), 165-170.
15. Farzaneh, M., Kiani, H., Sharifi, R., Reisi, M., & Hadian, J. (2015). Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest biology and technology, 109, 145-151.
16. Fathi, Z., Hassani, A., Ghosta, Y., Abdollahi, A., & Meshkatalsadat, M. H. (2012). The potential of thyme, clove, cinnamon and ajowan essential oils in inhibiting the growth of Botrytis cinerea and Monilinia fructicola. Journal of Essential Oil Bearing Plants, 15(1), 38-47.
17. Gandomi, H., Misaghi, A., Basti, A. A., Bokaei, S., Khosravi, A., Abbasifar, A., & Javan, A. J. (2009). Effect of Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese. Food and Chemical Toxicology, 47(10), 2397-2400.
18. Goepfert, J. (1980). Vegetables, fruits, nuts, and their products. Microbial ecology of foods, 2, 606-642.
19. Jalili Marandi, R., Hassani, A., Ghosta, Y., Abdollahi, A., Pirzad, A., & Sefidkon, F. (2011). Control of Penicillium expansum and Botrytis cinerea on pear with Thymus kotschyanus, Ocimum basilicum and Rosmarinus officinalis essential oils. Journal of Medicinal Plants Research, 5(4), 626-634.
20. Kashani Nezhad, M., & Kashani Nezhad, M. (2015). Design and analysis of tests in food industry using Design Expert 9. Publication of Gorgan University of Agricultural and Natural Resources, Gorgan, Iran. 1st edition. ISBN: 9789648926873- 9648926875. (In Farsi).
21. Jiang, C., Shi, J., Liu, Y., & Zhu, C. (2014). Inhibition of Aspergillus carbonarius and fungal contamination in table grapes using Bacillus subtilis. Food Control, 35(1), 41-48.
22. Kordsardouei, H., Barzegar, M., & Sahari, M. A. (2013). Application of Zataria multiflora Boiss. and Cinnamon zeylanicum essential oils as two natural preservatives in cake. Avicenna Journal of Phytomedicine, 3(3), 238-255.
23. Laidou, I., Thanassoulopoulos, C., & Liakopoulou‐Kyriakides, M. (2001). Diffusion of patulin in the flesh of pears inoculated with four post‐harvest pathogens. Journal of Phytopathology, 149(7‐8), 457-461.
24. Mahmoudvand, H., Mahmoudvand, H., Oliaee, R. T., Kareshk, A. T., Mirbadie, S. R., & Aflatoonian, M. R. (2017). In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice. Pharmacognosy magazine, 13(Suppl 3), S652.
25. Mohammadi, A., Hashemi, M., & Hosseini, S. (2015). The control of Botrytis fruit rot in strawberry using combined treatments of Chitosan with Zataria multiflora or Cinnamomum zeylanicum essential oil. Journal of Food Science and Technology, 52(11), 7441-7448.
26. Morales, H., Marín, S., Obea, L., Patiño, B., Doménech, M., Ramos, A. J., & Sanchis, V. (2008). Ecophysiological characterization of Penicillium expansum population in Lleida (Spain). International journal of food microbiology, 122(3), 243-252.
27. Nabavi-Pelesaraei, A., Rafiee, S., Mohtasebi, S. S., Hosseinzadeh-Bandbafha, H., & Chau, K.-w. (2018). Integration of artificial intelligence methods and life cycle assessment to predict energy output and environmental impacts of paddy production. Science of the Total Environment, 631, 1279-1294.
28. Naybandi Atashi, S., Mortazavi, S. A., Tabatabaei Yazdi, F., & Kouchaki, A. (2012). Investigation of synergystic effects of essential oils, Thymus volgaris, Mentha spp., and Ziziphora tenuir in inhibition of Escherichia coli O157:H7 as in vitro assay using Response Surface Methodology (RSM). Innovation in Food Science and Technology. 6(4), 19-30. (In Farsi).
29. Nikkhah, M., Hashemi, M., Najafi, M. B. H., & Farhoosh, R. (2017). Synergistic effects of some essential oils against fungal spoilage on pear fruit. International journal of food microbiology, 257, 285-294.
30. Oguaghamba, O., & Onyia, M. (2019). Modified and generalized full cubic polynomial response surface methodology in engineering mixture design. Nigerian Journal of Technology, 38(1), 52-59.
31. Pirbalouti, A. G., & Moalem, E. (2013). Variation in antibacterial activity of different ecotypes of Satureja khuzestanica Jamzad, as an Iranian endemic plant. Indian Journal of Traditional Knowledge, 12(4), 623-629.
32. Rasool, M., Malik, A., Alam, M. Z., Afzal, M., Alam, R., Arsalan, H. M., . . . Haque, A. (2018). Optimization of antibacterial activity of ethanolic extracts of Eucalyptus tereticornis and Nigella sativa: Response surface Methodology. Pakistan journal of pharmaceutical sciences, 31(4), 623-629.
33. Razavi, M. S., Asghari, A., Azadbakht, M., & Shamsabadi, H. (2018). Analyzing the pear bruised volume after static loading by Magnetic Resonance Imaging (MRI). Scientia Horticulturae. 229. 33-39. https://doi.org/10.1016/j.scienta.2017.10.01.
34. Saei-Dehkordi, S. S., Tajik, H., Moradi, M., & Khalighi-Sigaroodi, F. (2010). Chemical composition of essential oils in Zataria multiflora Boiss. from different parts of Iran and their radical scavenging and antimicrobial activity. Food and Chemical Toxicology, 48(6), 1562-1567.
35. Safari, N., Hemmati, R., Farzane, M., & Chegini, S. (2014). Study on three essential oils from Mentha piperita, Thymus daenensis and Satureja khuzistanica for controlling Penicillium expansum against of Apple Blue Mould. Applied Researches in Plant Protection, 3(1), 19-33. (In Farsi)
36. Spadaro, D., & Gullino, M. L. (2004). State of the art and future prospects of the biological control of postharvest fruit diseases. International journal of food microbiology, 91(2), 185-194.
37. Tajkarimi, M., Ibrahim, S. A., & Cliver, D. (2010). Antimicrobial herb and spice compounds in food. Food Control, 21(9), 1199-1218.
38. Valero, M., & Salmeron, M. (2003). Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. International journal of food microbiology, 85(1-2), 73-81.
39. Wisniewski, M. E., & Wilson, C. L. (1992). Biological control of postharvest diseases of fruits and vegetables: recent advances. HortScience, 27(2), 94-98.
40. Xing, Y., Li, X., Xu, Q., Yun, J., & Lu, Y. (2010). Antifungal activities of cinnamon oil against Rhizopus nigricans, Aspergillus flavus and Penicillium expansum in vitro and in vivo fruit test. International journal of food science & technology, 45(9), 1837-1842.
41. Yousefzadi, M., Riahi-Madvar, A., Hadian, J., Rezaee, F., Rafiee, R., & Biniaz, M. (2014). Toxicity of essential oil of Satureja khuzistanica: In vitro cytotoxicity and anti-microbial activity. Journal of immunotoxicology, 11(1), 50-55.
42. Zomorodian, K., Ghadiri, P., Saharkhiz, M. J., Moein, M. R., Mehriar, P., Bahrani, F., . . . Fani, M. M. (2015). Antimicrobial activity of seven essential oils from Iranian aromatic plants against common causes of oral infections. Jundishapur journal of microbiology, 8(2), 1-6.
)
