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گونه‎های جنس Bipolaris و Curvularia همراه گیاهان ذرت، سورگوم و نیشکر در ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج, گروه گیاه پزشکی

3 دانشیارگروه گیاهپزشکی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

4 استادیار بخش تحقیقاتی ذرت و گیاهان علوفه‎ای، موسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج، ایران

5 استادیار گروه ترویج و آموزش کشاورزی، مرکز آموزش عالی شهید باکری میاندوآب، دانشگاه ارومیه، میاندوآب، ایران

6 دانش آموخته کارشناسی ارشد گروه گیاه‎پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

به منظور مطالعه گونه‎های جنس‌های Bipolaris و Curvularia همراه گیاهان ذرت، سورگوم و نیشکر طی تابستان 1390 و 1391 نمونه‎برداری از اندام‎های هوایی گیاهان دارای علایم لکه‎برگی در مزارع مناطق مختلف کشور (استان‎های مازندران، گلستان، گیلان، اردبیل، زنجان، البرز، فارس و خوزستان) به عمل آمد. شناسایی گونه‌های قارچی بر اساس خصوصیات ریخت شناختی و اطلاعات توالی حاصل از نواحی ITS از DNA ریبوزومی صورت پذیرفت. بدین ترتیب تعداد 10 گونه شامل Bipolaris cynodontis (48 جدایه)، B. sorghicola (16 جدایه)، B. maydis (17 جدایه)، B. sorokiniana (هفت جدایه)، B. bicolor (چهار جدایه)، B. oryzae (دو جدایه) Curvularia spicifera (19 جدایه)، C. papendorfii (ده جدایه)، C. ellisii (دو جدایه) و C. hawaiiensis (یک جدایه) از گیاهان ذرت، سورگوم و نیشکر شناسایی شدند. آزمون بیماری‎زایی گونه‎ها روی گیاهچه‎های ذرت، سورگوم و نیشکر در مرحله چهار الی شش‌برگی صورت پذیرفت. در نهایت بیماری‏زایی گونه‎های B. sorokiniana و B. oryzae روی ذرت و گونه‎ B. sorghicola روی سورگوم اثبات شد. گونه‎های B. sorokiniana و B. oryzae برای اولین بار به عنوان بیمارگر روی ذرت در ایران گزارش می‌شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Bipolaris and Curvularia species associated with corn, sorghum and sugarcane in Iran and determination of their pathogenicity

نویسندگان [English]

  • Saadi Karami 1
  • Mohammad Javan Nikkhah 2
  • Khalil-Berdi Fotouhifar 3
  • Vahid Rahjoo 4
  • Abdollah Ahmadpour 5
  • Amin Alidadi 6
1 MSc Graduate, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 University of Tehran
4 Assistant Prof., Maize and Forage Crops Research Department, Seed and Plant Improvement Institute, Karaj, Iran
5 Higher Education Center Shahid Bakeri Miyandoab and Member of Ir an ’s National Elite Foundation, Urmia University, Urmia, Iran
6 MSc Graduate, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

In order to study the Bipolaris and Curvularia species associated with corn, sorghum and sugarcane, sampeling were performed from different regions of Iran (including Alborz, Ardabil, Fars, Gilan, Golestan, Khuzestan, Mazandaran and Zanjan provinces) during the summer of 2011 and 2012. Fungal species were identified based on morphological features and molecular data of ITS regions of ribosomal DNA. Eleven species including Viz. Bipolaris cynodontis (48 isolates), B. sorghicola (16 isolates), B. maydis (17 isolates), B. sorokiniana (seven isolates), B. bicolor (four isolates) and B. oryzae (two isolates), Curvularia spicifera (19 isolates), C. papendorfii (ten isolates), C. ellisii (two isolates), and C. hawaiiensis (one isolate) were identified. Pathogenicity test of the species were performed on four to six leaf stage of corn, sorghum and sugarcane seedlings. Finally, pathogenicity of B. maydis, B. sorokiniana and B. oryzae on corn and B. sorghicola on sorghum seedlings were confirmed. This is the first report of occurrence and pathogenicity of B. sorokiniana and B. oryzae on corn leaves in Iran.

کلیدواژه‌ها [English]

  • Cochliobolus
  • host
  • Morphology
  • phylogeny
  • Taxonomy
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