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بررسی ساختار و روابط تبارزایی پروتئین پوششی جدایه‌های ویروس موزائیک زرد لوبیا از مزارع باقلای ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری ویروس شناسی گیاهی دانشگاه ولی عصر(عج) رفسنجان

2 عضو هیات علمی/ دانشگاه ولی عصر رفسنجان

3 کارشناسی ارشد/ دانشگاه ولی عصر (عج) رفسنجان

4 هیات علمی

چکیده

ویروس موزائیک زرد لوبیا (Bean yellow mosaic virus) یکی از ویروس‌های متعلق به جنس پوتی‌ویروس، دارای دامنه میزبانی وسیع و پراکندگی جغرافیایی گسترده‌ای می‌باشد. این ویروس سالیانه خسارت بالایی به حبوبات مختلف از جمله باقلا در ایران وارد می‌کند. در این پژوهش13 جدایه‌ ویروس موزائیک زرد لوبیا از مزارع باقلای استان‌های مختلف ایران (استان‌های سیستان و بلوچستان، هرمزگان، کرمان، خوزستان، فارس، لرستان، ایلام، همدان، قزوین، زنجان، اردبیل و آذربایجان‌شرقی) جمع‌آوری گردید. ناحیه پروتئین پوششی جدایه‌های جمع‌آوری شده پس از توالی‌یابی با توالی پروتئین پوششی 178جدایه‌ منتخب از GenBank، مورد مقایسه قرار گرفت. توالی‌های نوکلئوتیدی جدایه‌های ایرانی مورد بررسی، به میزان 99-86 درصد با سایر توالی‌هایBYMV مشابهت داشتند. روابط تبارزایی جدایه‌های ویروس موزائیک زرد لوبیا پس از حذف جدایه‌های نوترکیب، با رسم درخت به روش Maximum Likelihood و بر اساس توالی نوکلئوتیدی ناحیه پروتئین پوششی مورد بررسی قرار گرفت. بر این اساس همه جدایه‌ها به جز سه جدایه AI38، PAC-1، BYMV-W در هشت گروه مونوفیلتیک قرار گرفتند. جدایه‌های ایرانی در دو گروه متمایز در کنار جدایه‌های باقلا، عدس، لوبیا، گلایول و آفتابگردان از کشورهای ژاپن، استرالیا، عراق و اسپانیا تقسیم‌بندی شدند. براساس نتایج بدست آمده، رابطه مستقیمی بین گروه‌بندی جدایه‌ها براساس بررسی تبارزایی توالی نوکلئوتیدی ناحیه پروتئین پوششی و منشا میزبانی و جغرافیایی مشاهده نشد. بررسی ساختار پروتئین پوششی در جدایه‌های ایرانی و سایر جدایه‌های موجود در GenBank نشان‌دهنده حفاظت‌شدگی بالای نواحی انتهایی کربوکسیل و ناحیه مرکزی پروتئین پوششی و متغیر بودن ناحیه ابتدای آمینی بود.

کلیدواژه‌ها


عنوان مقاله [English]

Structure and phylogenetic analysis of the coat protein of Bean yellow mosaic virus isolates from Iranian faba bean farms

نویسندگان [English]

  • Ali Baradar 1
  • Ahmad Hosseini 2
  • somaye abdani babaki 3
  • Samin Alsadat Hosseini Farhangi 4
1 PhD student of Agricultural and Plant Protection, Vali-e-Asr University of Rafsanjan
2 Plant Protection, Vali-e-Asr University of Rafsanjan
3 M.Sc. / Rafsanjan Valiasr University
4 Plant Protection. Vali-e-Asr University of Rafsanjan
چکیده [English]

Bean yellow mosaic virus, a species of the genus Potivirus, has a wide host range and a broad geographical distribution. BYMV causes high annual economic damage in various legumes such as faba beans in Iran. In this study, 13 BYMV isolates were collected from faba bean fields of different provinces of Iran (Sistan and Baluchestan, Hormozgan, Kerman, Khuzestan, Fars, Lorestan, Ilam, Hamadan, Ghazvin, Zanjan, Ardabil, East Azerbaijan). The coat protein (CP) region of the collected isolates was sequenced and then compared with the CP sequence of 178 isolates available in GenBank. The selected Iranian sequences showed 86-99% nucleotide sequence identities with other BYMV isolates. Phylogenetic relationships based on CP nucleotide sequences were estimated using the Maximum Likelihood method, after removing all recombinant sequences. Accordingly, all isolates excluding three isolates, AI38, PAC-1, BYMV-W were placed in eight monophyletic groups. Iranian isolates were located in two distinct groups, along with broadbean, lentil, bean, gladiolus and sunflower isolates from Japan, Australia, Iraq and Spain. According to the results there is no significant relation among clustering of BYMV isolates based on phylogenetic analysis of CP sequences and original host and country. The CP structure analysis of Iranian isolates and other selected isolates from GenBank revealed conservation of the C-terminus and the central region of the coat protein and the variation of the N-terminus.

کلیدواژه‌ها [English]

  • Viral disease
  • PCR
  • phylogeny
  • motif
  • recombination
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