c3518cb17d976b8

بررسی بیمارگری دو سویه از قارچ Beauveria bassiana (Bals.) Vuill روی لارو برگخوار چمن Spodoptera cilium Guenee در شرایط آزمایشگاه و مزرعه‌

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری حشره شناسی گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه شهید چمران اهواز، اهواز، ایران

2 استاد گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه شهید چمران اهواز، اهواز، ایران

3 استادیار گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه شهید چمران اهواز، اهواز، ایران

4 دانشیار گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

پروانه برگخوار چمن با نام علمی Spodoptera cilium (Guenee 1852) یکی از آفات چمن در فضای سبز مناطق جنوبی ایران و بسیاری از کشورهای جهان است. این تحقیق به منظور بررسی کارآیی دو سویه ایرانی قارچ Beauveria bassiana Vuill (Bals.) شامل IRAN440C و IRAN441C روی لاروهای S. cilium در شرایط آزمایشگاهی و مزرعه‌ صورت گرفت. لاروها در دمای۱±۲۵ درجه سلسیوس، رطوبت نسبی ۵±۶۰ درصد و تناوب نوری ۱۶:۸ ساعت روشنایی: تاریکی روی برگ چمن پرورش یافتند. اثر غلظت‌های مختلف دو سویه قارچ بر لارو های سن دوم و چهارم، با دو روش غوطه‌وری لاروها و آغشته‌سازی برگ چمن با سوسپانسیون اسپور انجام شد. نتایج نشان داد که در هر دو سن لاروی، درصد تلفات با افزایش غلظت اسپور، افزایش می‌یابد. بر اساس میزان تلفات ایجاد شده در دو مرحله، در هر دو روش، بیشترین درصد تلفات تجمعی (۱/۵۱ درصد) در غلظت ۱۰۶×۵ اسپور در میلی‌لیتر مشاهده شد. با افزایش غلظت سوسپانسیون کاهش معنی‌داری در مدت زمان لازم برای از بین بردن ۵۰ درصد جمعیت لاروها مشاهده شد. نتایج ما نشان داد که سویه IRAN440C قارچ B. bassiana در غلظت ۱۰۶×۵ اسپور در میلی‌لیتر موثرترین سویه از لحاظ زهرآگینی و ایجاد درصد تلفات بالا در جمعیت لاروهای برگخوار چمن شناخته شد. بنابراین، این سویه میتواند در برنامه‌های کنترلی کرم برگخوار چمن در فضای سبز شهری مورد استفاده قرار بگیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation the pathogenicity of two isolates of Beauveria bassiana Vuill (Bals.) on Spodoptera cilium Guenee larvae under laboratory and field conditions

نویسندگان [English]

  • Marziyeh Hatami 1
  • Ali asghar Seraj 2
  • Mehdi Mehrabi-Koushki 3
  • Masumeh Ziaee 4
1 PhD student in Entomology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Professor Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 ,Associate Professor Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

Spodoptera cilium (Guenee 1852) is one of the grass pests in green space in the southern regions of Iran and many countries around the world. This study was conducted to evaluate the effectiveness of the two Iranian isolates of Beauveria bassiana (Bals.)Vuill including IRAN440C and IRAN441C on S. cilium larvae under laboratory and field conditions. The larvae were reared at 25±10 C, a relative humidity of 60±5%, and a 16:8 h (L:D) photoperiod on grass leaves. The effect of different concentrations of two fungal isolates on second and third larval instars was performed by two methods of larval dipping and grass leaf immersion with spore suspension. The results showed that in both larval instars, the percentage of mortality increased with increasing spore concentration. Based on the mortality rate for both stages, in both methods, the highest percentage of cumulative mortality (51.1%) was observed at a concentration of 5×106 spores of fungi per mL. As the concentration of the suspension increased, a significant decrease in the time required to mortality 50% of the larval population was observed. Our results indicated that the IRAN440C strain of B. bassiana at 5×106 spores of fungi per mL concentration was the most effective strain in terms of toxicity and high mortality in the population of S. cilium. Therefore, this strain can be used in control programs of grasslawn armyworm in urban green space.

کلیدواژه‌ها [English]

  • Biocontrol
  • entomopathogen
  • mycosis
  • pathogenicity
  • Spodoptera cilium

مراجع

  1. Abood, F., Bajwa, G. A., Ibrahim, Y. B., and Sajap, A. S. (2010). Pathogenicity of Beauveria bassiana against the Tiger moth, Atteva sciodoxa (Lepidoptera: Yponomeutidae). Journal of Entomology, 7(1), 19-32.
  2. Asadpour, M., Zafari, D., and Zare, R. (2010). Hyphomycetes fungi isolated from insects and their pathogenic effects on Colorado beetle in Hamedan. Journal of Plant Protection (Agricultural Sciences and Industries), 24(4), 465-470. (In Farsi).
  3. Barbarin, A. M., Jenkins, N. E., Rajotte, E. G., and Thomas, M. B. (2012). A preliminary evaluation of the potential of Beauveria bassiana for bed bug control. Journal of Invertebrate Pathology, 111 (1), 82–85. 
  4. Baskar, K., and Ignacimuthu, S. (2012). Antifeedant, larvicidal and growth inhibitory effects of ononitol monohydrate isolated from Cassiatora L. against Helicoverpa armigera (Hub.) and Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). Chemosphere, 88, 384–388.
  5. Brown, E. and Dewhurst C. (1975). The genus Spodoptera (Lepidoptera, Noctuidae) in Africa and the near east. Bulletin of Entomological Research, 65(2), 221-262.
  6. Demirezer, P. (2006). Balcalı (Adana)’ da farklı habitatlardaki gece aktif Lepidoptera türleri ve biyolojik çeşitliliği üzerinde araştırmalar.Cukurova Universitesi Fen Bilimleri Enstitusu [Studies on lepidopteran species (moths) in different habitats and their biodiversity in Balcali (Adana)] (Unpublished master’s thesis). Adana, Turkey.
  7. Dhawan, M. and Joshi N. (2017). Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae Brazilian Journal of Microbiology, 48(3), 522-529.
  8. Donald, G., and McNeil, Jr. (2005). Fungus Fatal to Mosquito May Aid Global War on Malaria. The New York Time.

9.     El Husseini, M. M. (2019). Effect of the fungus, Beauveria bassiana (Balsamo) Vuillemin, on the beet armyworm, Spodoptera exigua (Hübner) larvae (Lepidoptera: Noctuidae), under laboratory and open field conditions. Egyptian Journal of Biological Pest Control, 29, 52-57.

  1. Fibiger, M., and Hacker, H. (2007). Amphipyrinae, Condicinae, Eriopinae, Xyleninae (PART) Noctuidae Europaeae. Vol. 9. Entomologycal Press, Soro, Denmark, 358 pp.
  2. Gulcua, B., Ulugb, D., Hazirc, C., Karagozd, M., and Hazirb, S. (2014). Biological control potential of native entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) against Spodoptera cilium (Lepidoptera: Noctuidae) in turfgrass. Biocontrol Science and Technology, 24(8), 965-970.
  3. Hazir, S. Kaya, H. K., Stock, S. P., and Keskin, N. (2003). Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turkish Journal of Biology, 27, 181–202.
  4. Heinrichs, E. A., and Barrion, A. T. (2004). Rice-feeding insects and selected natural enemies in West Africa: Biology, ecology, identification. Los Baños: International Rice Research Institute and Abidjan (Côte d’Ivoire): WARDA–The Africa Rice Center. 243 p.
  5. Indriyanti, D. R. Mahmuda, S. Slamet, M. (2017). Effect of Beauveria bassiana doses on Spodoptera litura International Journal of Scientific and Technology Research, 6(09), 206-210.
  6. Ismail, M., W. Wakil, N. H. Bashir, W. Hassan, and U. Muhammad. 2017. Entomocidal effect of entomopathogenic fungus Beauveria bassiana and new chemistry insecticides against Spodoptera litura (Fabricius)(Noctuidae: Lepidoptera) under controlled conditions. International Journal of Agriculture Innovations and Research, 5, 2319-1473
  7. Kaur, S., H. P. Kaur, K. Kaur, and Kaur, A. (2011). Effect of different concentrations of Beauveria bassiana on development and reproductive potential of Spodoptera litura (Fabricius). Journal of Biopesticides, 4, 161.
  8. Kitching, I. J., and Rawlins, J. E. (1999). The Noctuoidea. In: Kristensen, N.P. (Ed.), Handbook of Zoology: Lepidoptera: Moths and butterflies (Vol.1.). pp. 355-401.
  9. Klingen, I., Eilenberg, J., and Meadow, R. (2002). Effects of farming system, field margins and bait insect on the occurrence of insect pathogenic fungi in soils. Agriculture Ecosystem, and Environment, 91, 191–198.
  10. Kravchenko, V. D., Fibiger, M., Mooser, J., Junnila, A., and Müller, G. C. (2008). The tribes prodeniini and caradrinini of israel (Lepidoptera: Noctuidae, Xyleninae). SHILAP Revista de Lepidopteralogia, 36, 133–143.

20. Malarvannan, S., Murali, P. D., Shanthakumar, S. P., Prabavathy, V. R., and Nair, S. (2010). Laboratory Evaluation of the Entomopathogenic fungi, Beauveria bassiana Against the Tabacco Caterpillar, Spodoptera litura (Fab.)(Noctuidae: Lepidoptera). Journal of Biopesticides, 3(1), 126-131.

21. Mantzoukas, S. and Eliopoulos P. A. (2020). Endophytic entomopathogenic fungi: A valuable biological control tool against plant pests. Applied Sciences, 10(1), 360-373.

22. Mekhlif, A. F. (2007). Efficacy of enriched melia and zedarach L. extract on immature stages of the pest Spodoptera cilium latebrosc (Guerine) (Lepidoptera: Noctuidae). Tikrit Jourrtnl of Pltarnmceulical Sciences, 3(l), 63 – 65.

23. Moorthi, P. V., C. Balasubramanian, and Kubendran, T. (2011). Efficacy of local isolates of Beauveria bassiana against Spodoptera litura (F.)(Lepidoptera: Noctuidae). Journal of Biological Control, 25, 22-25.

24. Ngangambe, M. H., and Mwatawala, M. W. (2020). Effects of entomopathogenic fungi (EPFs) and cropping systems on parasitoids of fall armyworm (Spodoptera frugiperda) on maize in eastern central, Tanzania. Biocontrol Science and Technology, 30, 418-430.

  1. Ortiz-Urquiza, A. Luo, Z. and Keyhani, NO. (2015). Improving mycoinsecticides for insect biological control. Applied Microbiology and Biotechnology, 99(3), 1057–1068.
  2. Ortiz-Urquiza, A. Riveiro-Miranda, L. Santiago, A. ´lvarez, C. and QuesadaMoraga, E. (2010). Insect-toxic secreted proteins and virulence of the entomopathogenic fungus Beauveria bassiana. Journal of Invertebrate Pathology, 105(3), 270–278.
  3. Petlamul, W., and Prasertsan, P. (2012). Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia production, radial growth and enzyme activity. Mycobiology, 40, 111-116.
  4. Quesada-Moraga, E. and Vey, A. (2003). Intra-specific variation in virulence and in vitro production of macromolecular toxins active against locust by Beauveria bassiana and effects of in vivo and in vitro passage on these factors. Biocontrol Science and Technology, 13, 323–340.
  5. Ramanujam, B., B. Poornesha, and A. Shylesha. 2020. Effect of entomopathogenic fungi against invasive pest Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae) in maize. Egyptian Journal of Biological Pest Control, 30, 1-5.
  6. Safavi, A., Kharazi, A., Rasolian, Gh, and Bandani, A. (2009). Multiple insect disease of insect fungus Beauveria bassiana (Bals.) Vuill on European corn stem borer Ostrinia nubilalis (Lepidoptera: Pyralidae). Journal of Agricultural Science and Technology, 12 (1), 21-13. In Farsi.
  7. Sánchez-Rodríguez, A. R., S. Raya-Díaz, Á. M. Zamarreño, J. M. García-Mina, M. C. del Campillo, and Quesada-Moraga, E. (2018). An endophytic Beauveria bassiana strain increases spike production in bread and durum wheat plants and effectively controls cotton leafworm (Spodoptera littoralis) larvae. Biological control, 116, 90-102.
  8. Seraj, A. A. (2011). Principles of Plant Pests Control. Ayneband, A. and Ashouri, A. (Eds.), Shahid Chamran Universiy of Ahvaz. 754 p.
  9. Shahriari Nejad, S. (2016). Study of Noctuidae phonon in parts of Khuzestan and Kerman and identification of important pest species by barcode DNA method. PhD Thesis, Department of Agricultural Entomology, Shahid Chamran University of Ahvaz. Pages 112-113. In Farsi
  10. Shehzad, M., Tariq, M., Mukhtar, T., Gulzar, A. (2021). On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). Egyptian Journal of Biological Pest Control, 31, 86-93.
  11. Tanada, Y., and Kaya, H. K. (1993). Insect pathology. Academic Press, London, pp 319–385
  12. Taylor, J. E., and Riley D. G. (2008). Artificial infestations of beet armyworm, Spodoptera exigua (Lepidoptera: Noctidae) used to estimate an economic injury level in tomato. Crop Protection, 27, 268–274.
  13. Unlu, L., and Kornosor, S. (2003). Sanliurfa ilinde saptanan noctuidae (lepidoptera) familyasi turleri ve morfolojik ozellikleri [Determination of the species of noctuidae (lepidoptera) family in Sanliurfa and morphological characteristics]. Harran University Journal of Faculty of Agriculture, 7, 19 –28.
  14. Vuillemin, P. (1912). Beauveria, nouveau genre de Verticilliacées. Bulletin de la Société Botanique de France 59, 34–40
  15. Wibawanti, R., and Herminanto, D. (2010). Potensi jamur entomopagen Beauveria bassiana Untuk mengendalikan ulat grayak (Spodoptera litura F.) potency of the fungus Beauveria bassiana Vuill. for controlling armyworm (Spodoptera litura F.). Jurnal Pembangunan Pedesaan, 10(1), 39-46
  16. Wraight, S. P., M. E. Ramos, P. B. Avery, S. T. Jaronski, and Vandenberg, J. D. (2010). Comparative virulence of Beauveria bassiana isolates against lepidopteran pests of vegetable crops. Journal of Invertebrate Pathology, 103, 186-199.
  17. Zibaee, I., A. R. Bandani, and Sendi, J. J. (2013). Pathogenicity of Beauveria bassiana to fall webworm (Hyphantria cunea)(Lepidoptera: Arctiidae) on different host plants. Plant Protection Science, 49, 169-176.
  18. Zimmermann, G. (2007). Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science and Technology, 17, 553–596.

 

دانش گیاهپزشکی ایران
دوره 52، شماره 2
بهمن 1400
صفحه 15-24

فایل ها

سابقه مقاله

  • تاریخ دریافت: 29 شهریور 1399
  • تاریخ بازنگری: 28 خرداد 1400
  • تاریخ پذیرش: 08 تیر 1400

هم رسانی

ارجاع به این مقاله

آمار