c3518cb17d976b8

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

2 گروه شیمی فیزیک، دانشکده شیمی، دانشگاه بوعلی‌سینا، همدان

چکیده

استفاده از اسانس‌های گیاهی در برنامه‌های مدیریتی آفات به عنوان جایگزین یا مکمل حشره‌کش‌های شیمیایی، ایمن و نویدبخش به نظر می‌رسد. چنین امکانی منوط به رفع نواقصی همچون ناپایداری شیمیایی، تبخیرپذیری و حلالیت کم اسانس‌های گیاهی در آب است. استفاده از نانوفرمولاسیون‌ها با امکان رهایش کنترل شده یک راه‌کار مؤثر در جهت رفع نواقص فوق به شمار می‌آید. پژوهش پیش‌رو با هدف شناسایی ترکیبات مؤثر اسانس آزاد پونهMentha longifolia و نانوفرمولاسیون آن با استفاده از روش کروماتوگرافی گازی- طیف‌سنجی جرمی و تهیه نانو سیلیکای حاوی اسانس پونه به روش سل- ژل و بررسی خصوصیات فیزیکوشیمیایی آن در جهت امکان‌‌سنجی کاربرد در برنامه‌های مدیریت تلفیقی آفات انجام شده است. نتایج نشان دهنده بیشترین فراوانی کتون‌ها (پیپریتنون 32% و پولگون 44/17%) و منوترپن‌های اکسیژن‌دار (01/24%) در بین اجزاء سازنده اسانس بود. بهترین ترکیب اجزاء و شرایط انجام واکنش نیز از نظر اندازه ذرات نانوفرمولاسیون تهیه شده و کارایی کپسوله شدن عبارت بود از تترا اتیل ارتو سیلیکات (TEOS) (5/0 میلی‌لیتر)، ستیل تری متیل آمونیوم برماید (CTAB) (08/0 گرم)، اسانس (1 ملی‌لیتر)، اتانول (13 میلی‌لیتر)، آمونیاک 25% (5/0 میلی‌لیتر)، استفاده از همزن التراسونیک به مدت 2 ساعت در دمای اتاق و در نهایت سانتریفیوژ به مدت 5 دقیقه (rpm 15000). از ویژگی‌های نانو‌سیلیکای حاوی اسانس پونه، تهیه شده با استفاده از روش سل- ژل، قطر متوسط ذرات 80-66 نانومتر، پتانسیل زتای37-، شاخص پراکندگی 2/0 و راندمان ریزپوشانی 64/79 درصد بوده است.

کلیدواژه‌ها

عنوان مقاله [English]

Mentha longifolia nanoencapsulated essential oil: Its synthesis and physico-chemical properties

نویسندگان [English]

  • Maryam Malekmohammadi 1
  • Moein Jaafaripoorderagahi 1
  • AmirAbbas Rafati 2

1 Department of Plant Protection, Faculty of Agriculture, Bu- Ali Sina University, Hamedan, Iran

2 Department of Physical Chemistry, Faculty of Chemistry, Bu- Ali Sina University, Hamedan, Iran

چکیده [English]

Essential oils app;ication in integrated pest management as alternative or complementary to chemical insecticides appears quite safe and promosing. However the chemical instability, volatility and poor water solubility remain the main disadvantages of essential oils and must be resolved before being incorporated into pest management systems. Incorporation of essential oils into a controlled-release nanoformulation is an efficient approach to overcome these limitations. So the present study aimed to analyze chemical constituents of Mentha longifolia free and nanoencapsulated essential oils by gas chromatography–mass spectrometry (GC–MS) and describe a practical approach to synthesize nano hollow silica spheres by sol-gel method as a Mentha longifolia essential oil carrier. GC–MS analysis resulted in the detection of the 43 components, piperitenone (%32), piperitenone oxide (%24.01) and pulegone (%17.44) were the three most representative constituents. The success of M. longifolia essential oil encapsulated was characterized based on some parameters including particle size, zeta potential, polydispersity index (PDI) and encapsulation efficiency. The Optimised Reaction mixture consisting of TEOS (0.5 ml), CTAB (0.08 g), essential oil (1 ml), ethanol (13 ml), 25% ammonia (0.5 ml), 2 hours of ultrasonication at room temperature and emulsification at 15000 rpm for 5 min, resulted in particle size between 66 to 80 nm, a polydispersity index of 0.2, zeta pontencial of −37 mV and an EE of 79.64%.

کلیدواژه‌ها [English]

  • Essential oil
  • mint
  • Encapsulation
  • Sol- Gel
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