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نوع مقاله : مقاله پژوهشی

نویسندگان

1 مرکز تحقیقات کشاورزی و منابع طبیعی کرمانشاه

2 استادیار پژوهشی بخش تحقیقات کنترل بیولوژیک، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران.

3 دانشیار پژوهشی، بخش تحقیقات به نژادیموسسه تحقیقات چغندر قند، کرج، ایران

4 دانشیار پژوهشی، بخش تحقیقات چغندر قند، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی کرمانشاه

چکیده

در این تحقیق، کارآیی ماده بیولوژیکی رویین 1 بر اساس باکتریBS 106 Bacillus subtilis در کنترل بیماری پوسیدگی ریشه چغندرقند با عامل Rhizoctonia solani بررسی شد. آزمایش به صورت طرح آماری کرت‌های خرد‌شده در قالب بلوک‌های کامل تصادفی در دو مزرعه تحقیقاتی در شهرستان کرمانشاه در سال زراعی 98-1397 انجام گردید. عامل اصلی شامل شش تیمار به ترتیب تیمار بذر با ماده بیولوژیکی رویین 1 با غلظت‌های 100، 200 و 400 گرم ماده تجارتی برای چهار کیلوگرم بذر مصرفی در هکتار، تیمار بذر با رویین 1 با استفاده از دستگاه پوشش‌دهی بذر با محلول حاوی200 گرم ماده تجارتی در چهار کیلوگرم بذر، تیمار کربوکسین تیرام و شاهد. عامل فرعی عبارت از محلول‌ریزی در دو سطح شامل کاربرد و عدم استفاده از آن بود. نتایج این تحقیق نشان داد که عامل اصلی به صورت پوشش بذر به صورت‌های مختلف تاثیری در کاهش شدت بیماری و یا افزایش محصول نداشت، اما عامل فرعی به صورت محلول‌ریزی سبب کاهش شدت بیماری و افزایش عملکرد در سطح آماری یک درصد شد. با اعمال تیمار محلول‌ریزی ماده تجارتی رویین 1 به میزان 200 گرم در هکتار در دو مرحله 45 و 90 روز بعد از کاشت، میانگین شاخص بیماری از 4/4 (میانگین تیمار شاهد) به 58/3 (میانگین تیمار محلول‌ریزی) رسید. در نتیجه شاخص بیماری به میزان 82/0 واحد (معادل تقریبی2/8 درصد میزان آلودگی) کاهش یافت. همچنین میانگین محصول از 39560 کیلوگرم در تیمار شاهد به 48080 در تیمار محلول ریزی افزایش یافت که معادل 72/17 درصد افزایش محصول در هکتار است.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the Rouin1 biological fungicide in management of Rhizoctonia root rot of sugar beet

نویسندگان [English]

  • Mahyar Sheikholeslami 1
  • Shahram Naeimi 2
  • Seyed Bagher Mahmoudi 3
  • Ali Jalilian 4

1 Agricultural and natural resources research center of Kermanshah

2 Prof. Assistant for biological control Research Department, Iranian Research institute of Plant Protection, AREEO, Tehran, Iran.

3 Prof. Associate for Research Department of plant breeding, Sugar beet seed institute, Karaj, Iran.

4 Prof. Associate for Sugar beet Research Department, Kermanshah Agricultural and Natural Resources Research and Education Center, AREEO, Kermanshah, Iran

چکیده [English]

In this study, the efficacy of the biological material named as Rouin1 based on Bacillus subtilis BS106 in controlling Rhizoctonia root rot disease of sugar beet was evaluated. The experiment was carried out as split plots in the pattern of randomized complete block design in two research farms in Kermanshah county. The major factor was seed coating with Rouin1 at various conditions. The minor factor was irrigating and not irrigating the plots with Rouin1 solution. All of the plants were inoculated with R. solani RS 124 inoculum 8 weeks after planting. The results of this study showed that major factor as seed coating had no effect on disease severity or crop yield in different forms, but minor factor as irrigating with bacterial solution reduced disease severity and increased crop yield at 1% probability level. By applying irrigation treatment of Rouin1 commercial material at the rate of 200 g/ha at two stages of 45 and 90 days after planting, the average disease index decreased from 4.4 (average control treatment) to 3.58 (average irrigation treatment). As a result, the disease index decreased by 0.82 units (approximately equivalent to 8.2% of the infection rate). Also, the average yield increased from 39,560 kg in the control treatment to 48080 in the irrigation treatment, which is equivalent to 17.72% of production increasing per hectare.

کلیدواژه‌ها [English]

  • Biological control
  • Rhizoctonia root rot
  • seed treatment
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