c3518cb17d976b8

شناسایی منابع مقاومت به عامل بیماری زنگ قهوه‌ای (Puccinia triticina Erikss.) در ژنوتیپ‌های گندم بومی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح نباتات دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران

2 استاد اصلاح نباتات دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران

3 اشتادیار موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

4 دانشیار اصلاح نباتات دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران

5 استاد یار گروه زراعت دانشکده کشاورزی دانشگاه ارومیه

چکیده

شناسایی منابع ژن‌های مقاومت گیاهچه‌ای و گیاه کامل برای هرمی کردن ژن‌ها، آرایش ژنی و ایجاد ارقام گندم دارای مقاومت پایدار، برای کنترل زنگ قهوه‌ای گندم، با عامل .Puccinia triticinea Erickss امری ضروری است. در این پژوهش تعداد 218 ژنوتیپ گندم بومی ایران که از مناطق مختلف جمع‌آوری شده بود، در مزرعه آزمایشی ایستگاه تحقیقات کشاورزی گلستان اهواز طی سال 99-1398 به صورت خطوط یک متری کشت شده و با پاتوتیپ‌های شایع زنگ قهوه‌ای خوزستان شامل LR-98-8، LR-98-14، LR-94-5 و LR-97-4 تیمار شدند. واکنش گیاهچه‌ای نیز در گلخانه‌های واحد بیماری‌شناسی غلات موسسه اصلاح و تهیه نهال و بذر کرج با پنج پاتوتیپ شایع ایران در قالب طرح بلوک‌های کامل تصادفی با دو تکرار انجام شد. برای ارزیابی مقاومت از شاخص‌هایی تیپ آلودگی، سطح زیر منحنی پیشرفت بیماری، شدت نهایی آلودگی و ضریب آلودگی استفاده شد. نتایج نشان داد که به ترتیب 10 و هفت درصد از ژنوتیپ‌ها در شرایط گلخانه‌ای و مزرعه‌ای مقاومت نشان دادند. در مرحله گیاه کامل واکنش 23 ژنوتیپ به صورت نیمه‌مقاوم (MR: moderately resistant) و 14 ژنوتیپ به‌صورت نیمه‌حساسMS: moderately susceptible) ) بود. ضریب همبستگی بین شدت نهایی آلودگی، با مقادیر ضریب آلودگی و سطح نسبی زیر منحنی پیشرفت بیماری (rAUDPC)، به ترتیب 989/0 و 949/0 بود. در نهایت 30 ژنوتیپ با خصوصیات مورد نظر یعنی شدت نهایی آلودگی 40-0 درصد، ضریب آلودگی 40-0 وrAUDPC بین 30-0 درصد به‌ عنوان ژنوتیپ‌های مطلوب انتخاب شدند. تجزیه خوشه‌ای ژنوتیپ‌های مورد نظر را در چهار گروه حساس (S: Sensetive)، نیمه‌حساس (MS)، نیمه‌مقاوم (MR) و مقاوم (R: Resistance) طبقه‌بندی کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Identification sources of resistance for leaf rust (Puccinia triticina Erikss.) in Iranian wheat genotypes

نویسندگان [English]

  • Saba Delfan 1
  • MohammadReza Bihamta 2
  • Seyed Taha Dadrezaei 3
  • Alireza Abbasi 4
  • Hadi Alipour 5
1 PhD student in Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran
2 Professor of Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran
3 Department of Center Research. Seed and Plant Improvement Institute. Agricultural Research, Education and Extension Organization (AREEO) Karaj, iran
4 Associate Professor of Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran
5 Assistant Professor, Department of Agriculture, Faculty of Agriculture, Urmia University
چکیده [English]

Identification the sources of seedling and adult plant resistance genes is important for gene pyramiding, gene deployment, and developing durable wheat cultivars to control the leaf rust with Puccinia triticinea Erickss agent. In this study, 218 Iranian wheat genotypes collected from different regions were cultivated in one-meter lines in the experimental farm of Golestan Agricultural Research Station in Ahwaz during 2019-2020 and treated with Khouzestan leaf rust prevalent pathotypes including LR-98-8, LR-98-5, LR-94-5 and LR-9-4. Seedling reactions with five prevalent pathotypes were carried out in a randomized complete block design with two replications in the greenhouses of the Cereal Pathology Unit of Seed and Plant Breeding Institute, Karaj. To evaluate resistance, we used Infection Type (IT), AUDPC (Area Under Disease Progression Curve), Final Disease Severity(FDS) and Coefficient Infection (CI) indices. The results showed that 10 percent and 7 percent of the genotypes are resistant in greenhouse and field conditions, respectively. In the adult plant stage, the responses of 23 genotypes were moderately resistant (MR) and 14 genotypes were moderately susceptible (MS). The correlation coefficients between the final disease severity, with the coefficient infection and rAUDPC were 0.989 and 0.949, respectively. Finally, 30 genotypes with the desired characteristics, ie final disease severity between 0-40%, coefficient of infection between 0-40 and rAUDPC between 0-30% were selected as the suitable genotypes. Cluster analysis classified the genotypes into four groups: susceptible (S), moderately susceptible (MS), moderately resistant (MR) and resistant (R).

کلیدواژه‌ها [English]

  • Wheat Leaf Rust
  • Area Under Disease Progress Curve (AUDPC)
  • Seedling Resistance
  • Adult Plant Resistance

مراجع

  1. Ali, S., Shah, S. J. A., Raman, I. K. H., Maqbool, K., & Ullah, W. (2009). Partial resistance to yellow rust in introduced winter wheat germplasm at the north of Pakistan. Australian Journal of Crop Science, 3(1), 37-43.‏
  2. Alipour, H., Bihamta, M. R., Mohammadi, V., & Peyghmbari, S. A. )2017(. Evaluation of genetic variability of agronomic traits in Iranian wheat landraces and cultivars. Journal of Crop Breeding, 9(22), 168-177 (In Farsi).
  3. Alipour, H., Bihamta, M. R., Mohammadi, V., Peyghambari, S. A., Bai, G., & Zhang, G. (2017). Genotyping-by-sequencing (GBS) revealed molecular genetic diversity of Iranian wheat landraces and cultivars. Frontiers in plant science, 8, 1293.‏
  4. Amin, K. P., & Robert, F. P. )2006(. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica, 149(3), 327–342
  5. Bamdadeian, A. (1993). Evaluation of physiological race of rusts of grass and their modification in Iran. Institute of Evaluation Pests and Plant Disease, Evin, Iran, 10p (In Farsi).
  6. Bonman, J. M., Babiker, E. M., Cuesta-Marcos, A., Esvelt-klos, K., Brown-Guedira, G., Chao, S., See, D., Chen, J., Akhunov, E., Zhang, J., Bockelman, H. E. & Gordon, T.C. (2015). Genetic diversity among wheat accessions from the USDA national small grains collection. Crop Science, 55(3), 1243-1253.
  7. Chen, X. M. (2013). Review article: High-temperature adult-plant resistance, key for sustainable control of stripe rust. American Journal of Plant Sciences, 4, 608–627.
  8. Dadrezaie, S. T., Afshari, F., & Patpoor, M. (2015). Evaluation of phenotypic resistance to rusts in some Iranian wheat genotypes in greenhouse and field conditions. Seed and Plant Improvement Journal. 1(31), 531-546 (In Farsi).
  9. Dehghan, M. A., Malihipour, A., & Khodarahmi, M. (2017). Evaluation of resistance of selected bread wheat genotypes of cereal research department of seed and plant institutes to fusarium head blight in field conditions. Journal of Applied Plant Protection, 6(1), 35-53 (In Farsi).
  10. Draz, I. S., Abou-Elseoud, M. S., Kamara, A. E. M., Alaa-Eldein, O. A. E., & El-Bebany, A. F. (2015). Screening of wheat genotypes for leaf rust resistance along with grain yield. Annals of Agricultural sciences, 60(1), 29-39.‏
  11. Dvorak, J., & Knott, D. R. (1990). Location of a Triticum speltoides chromosome segment conferring resistance to leaf rust in Triticum aestivum. Genome, 33(6), 892-897.
  12. Dyck, P. L. (1979). Identification of the gene for adult-plant leaf rust resistance in Thatcher. Canadian journal of plant science, 59(2), 499-501.‏
  13. Dyck, P. L., & Samboraski, D. J. (1979). Adult plant leaf rust resistance in PI 250413, an introduction of common wheat. Conadian Journal of Plant Science, 59(2), 329-332.
  14. Dyck, P. L., Kerber, E. R. & Lukow, O. M. (1987). Chromosome location and linkage of a new gene (Lr33) for reaction to Puccinia recondita in common wheat. Genome, 29(3), 463-466.
  15. Dyck, P. L., Samboraski, D. J, & Anderson, R. G. (1966). Inheritance of adult plant leaf rust resistance derived from the common wheat varieties Exchange and Frontana. Canadian Journal of Genetics and Cytology, 8(4), 665-671.
  16. Ebrahimyan, M., Nasrollahnezhad Ghomi, A., Null, K., & Ramezanpour, S. (2019). Evaluation of resistance to leaf rust at adult stage in some bread wheat cultivars. Journal of Plant Production Research. 26(3), 89-102 (In Farsi).
  17. Esfandeiari, A. (1948). The rusts of grass in Iran. Journal of Pests and Plant Disease, 4, 76-77 (In Farsi).
  18. Hei, N. B. (2017). Evaluation of wheat cultivars for slow rusting resistance to leaf rust (Puccinia trticina Eriks) in Ethiopia. African Journal of Plant Science, 11(2), 23-29.‏
  19. Hei, N., Shimelis, H. A., Laing, M., & Admassu, B. (2015). Assessment of Ethiopian wheat lines for slow rusting resistance to stem rust of wheat caused by Puccinia graminis f. sp. tritici. Journal of Phytopathology, 163(5), 353-363.‏
  20. Huerta-Espino, J., Singh, R., Crespo-Herrera, L., Villaseñor-Mir, H., Rodriguez-Garcia, M., Dreisigacker, S., Barcenas-Santana, D., & Lagudah, E. (2020). Adult plant slow rusting genes confer high levels of resistance to rusts in bread wheat cultivars from Mexico. Frontiers in Plant Science, 11:824, doi: 10.3389/fpls.2020.00824.
  21. Johnson, R. 1988. Durable resistance to yellow (stripe) rust in wheat and its implications in plant breeding: Breeding strategies for resistance to the rusts of wheat. (pp. 63-75). CIMMYT: Mexico.
  22. Kolmer, J. (2013). Leaf rust of wheat: pathogen biology, variation and host resistance. Forests, 4(1), 70-84.‏
  23. Li, G., Xu, X., Bai, G., Carver, B. F., Hunger, R., & Bonman, J. M. (2017). Novel sources of leaf rust resistance in winter wheat. Crop Science, 57(2), 865-876.‏
  24. McIntosh, R. A., Dubcovsky, J., Rogers, W. J., Morris, C., Appels, R., Xia, X. C., & AZUL, B. (2013). Catalogue of gene of symbols for Wheat: 2013-2014. In Proceedings of the 12th International Wheat Genetics Symposium, 7 September, Yokohama, Japan.‏
  25. McIntosh, R. A., Wellings, C. R., & Park, R. F. (1995). Wheat Rusts: an altas of resistance genes. Kluwer Academic Publishers, The Netherlands, 220pp.
  26. Mojerlou, S. H., Safaei, N., Abbasi_Moghaddam, A., & Shams Bakhsh, M. (2013). Evaluation of some Iranian wheat landraces resistance against stem rust resistance at seeding stage in the greenhouse. Plant Protection Journal, 35(4), 69-82 (In Farsi).
  • Nemati, Z., Mostowfizadeh-Ghalamfarsa, R., Dadkhodaie, A., Mehrabi, R., & Steffenson, B. J. (2020). Virulence of leaf rust physiological races in Iran from 2010 to 2017. Plant disease, 104(2), 363-372.‏
  1. Parlevliet, J., & Ommeren, A. (1975). Partial resistance of barley to leaf rust, Puccinia hordei. Relationship between Field Trails, Micro Plot Tests and Latent Period. Euphytica, 24(2), 293-303.
  2. Peterson, R. F., Campbell, A. B., & Hannah, A. E. (1948). A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian journal of research, 26(5), 496-500.‏
  3. Qureshi, N., Bariana, H., Kumran, V. V., Muruga, S., Forrest, K. L., Hayden, M. J., & Bansal, U. (2018). A new leaf rust resistance gene Lr79 mapped in chromosome 3BL from the durum wheat landrace Aus26582. Theoretical and Applied Genetics, 131(5), 1091-1098.‏
  4. Roelfs, A. P., Singh, R. P., & Saari, E. E. (1992). Rust disease of wheat: Concepts and Methods of Disease Management. CIMMIT, Mexico, 81pp.
  5. Safavi, S. A., Ahari, A., Afshari, F., Arzanlou, M .(2013). Slow rusting resistance in Iranian barley cultivars to Puccinia striiformis f. sp. hordei. Journal of Plant Protection Research. 53(1): 6-11.
  6. Safavi, S. A., Malihipour, A. (2020). Partial resistance of some wheat cultivars and candidate lines against stem rust (Puccinia graminis f. sp. tritici). Plant Protection (Scientific Journal of Agriculture), 43(1), 31-53 (In Farsi).
  7. Sapkota, S., Hao, Y., Johnson, J., Buck, J., Aoun, M., & Mergoum, M. (2019). Genome‐wide association study of a worldwide collection of wheat genotypes reveals novel quantitative trait loci for leaf rust resistance. The Plant Genome, 12(3), 1-14.‏
  8. Soliman, A. S., Heyne, E. G., & Johnston, C. O. (1963). Resistance to leaf rust in wheat derived from Chinese Aegilops umbellulata translocation lines 1. Crop Science, 3(3), 254-256.
  9. Sthapit, J., Newcomb, M., Bonman, J. M., Chen, X., and See, D. R. (2014). Genetic diversity for stripe rust resistance in wheat landraces and identification accessions with resistance to stem rust and stripe rust. Crop Science, 54(5), 2131-2139.
  10. Torabi, M., Nazari, K., & Afshari, F. (2001). Genetic of pathogenicicty of Puccinia recondita f.sp. tritici, the causal agent of leaf rust of wheat. Iranian Journal Agricultral Science, 32(3), 625-635 (In Farsi).
  11. Tsilo, T. J., Jin, Y., & Anderson, J. A. (2010). Identification of flanking markers for the stem rust resistance gene Sr6 in wheat. Crop science, 50(5), 1967-1970.‏
  12. Winzeler, M., Winzeler, H., & Keller, B. (1995). Endopeptidase polymorphism and linkage of the Ep‐D1c null allele with the Lrl9 leaf‐rust‐resistance gene in hexaploid wheat. Plant breeding, 114(1), 24-28.‏
  13. Xu, X., Bai, G., Carver, B. F., Shaner, G. E., & Hunger, R. M. (2005). Molecular characterization of slow leaf‐rusting resistance in wheat. Crop science, 45(2), 758-765.‏
  14. Zahravi, M., Afshari, F., Ebrahimnejad S. (2019). Study of genetic diversity of resistance to yellow rust in bread wheat, Modern Genetics Journal, 14(3), 263-274.
  15. Zhang, P., Li, X., Gebrewahid, T. W., Liu, H., Xia, X., He, Z., Li, Z. & Liu, D. (2019). QTL mapping of adult plant resistance to leaf and stripe rust in wheat cross SW8588/Thatcher using the wheat 55K SNP array. Plant Disease, 103(2), 3041-3049.
دانش گیاهپزشکی ایران
دوره 52، شماره 2
بهمن 1400
صفحه 115-133

فایل ها

سابقه مقاله

  • تاریخ دریافت: 12 دی 1399
  • تاریخ بازنگری: 18 اردیبهشت 1400
  • تاریخ پذیرش: 30 مرداد 1400

هم رسانی

ارجاع به این مقاله

آمار