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نوع مقاله : مقاله پژوهشی

نویسندگان

1 مربی پژوهشی، بخش تحقیقات چغندرقند، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج

2 دانشیار ،گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد،ایران

3 دانشیار، مؤسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

ویروس سوختگی سیاه برگ چغندرقند (Beet black scorch virus, BBSV) ازجمله ویروس‌های خاک‌برد چغندرقند است. در این تحقیق، 80 نمونه چغندرقند از مزارع خراسان رضوی و شمالی جمع‌آوری شد. پس از استخراج RNAی کل آن‌ها، ناحیه 3'UTR ژنوم ویروس به تعداد 315 نوکلئوتید با استفاده از واکنش زنجیره‌ای پلیمراز با رونویسی معکوس و بهره‌گیری از آغازگرهای اختصاصی رفت و برگشتی تکثیر شد. این ناحیه در شش جدایه مختلف پس از همسانه سازی، توالی‌یابی و در NCBI ثبت شدند. نتایج بررسی خویشاوندی این جدایه‌ها (بر اساس (3'UTR با کل جدایه‌های ثبت‌شده در بانک جهانی نشان داد که آن‌ها در دو گروه عمده I و II قرار می‌گیرند به‌طوری‌که هر یک به دو زیرگروه تقسیم می‌شوند. شش جدایه این بررسی با 23 جدایه از نقاط مختلف ایران در زیرگروه IA و چهار جدایه دیگر از خراسان در زیرگروه IB جای گرفتند. سه جدایه از کرمانشاه و یک جدایه از همدان (Ir-KSh5,Ir-KSh4 Ir-KSh6 و Ir-Ha2) در زیرگروه IIA قرار گرفتند. چهار جدایه از کرمانشاه Ir-KSh9,Ir-KSh8,Ir-KSh7) و Ir-KSh10) به همراه جدایه‌های چینی، اروپایی و آمریکایی زیرگروه IIB را تشکیل دادند. نتایج مقایسه نوکلئوتیدی توالی شش جدایه نشان داد که اگرچه تغییر نوکلئوتیدی مهم در موقعیت 3477 (یوریدین بجای گوانین) صورت نگرفته است، اما در موقعیت 3398 آدنین جایگزین گوانین و در موقعیت 3393 فقط در جدایه فریمان (MW274750) یوریدین بجای سیتوزین جایگزین شده است. نتایج مقایسه میانگین شباهت نوکلئوتیدی ناحیه 3'UTR این جدایه‌ها نشان داد که با جدایه‌های ایرانی (به غیر از جدایه-های کرمانشاه با 52/92 درصد) در دامنه بین 75/98-37/97 درصد مشابهت دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of genetic variation of 3′UTR region of Beet black scorch virus in Khorasan isolates with the other isolates from Iran and the world

نویسندگان [English]

  • Jamshid soltani Idliki 1
  • mohsen mehrvar 2
  • seyed bagher mahmoudi 3

1 Sugar Beet Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran.

2 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Associate Professor, Sugar Beet Seed Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karadj, Iran

چکیده [English]

Beet black scorch virus is one of the soil-borne sugar beet viruses in Iran. In this research work, we collected 80 samples of sugar beet farms in Razavi and Northern Khorasan Provinces. After extraction of their total RNA, the 3'UTR region of the virus genome was amplified to 315 nucleotides using reverse transcription polymerase chain reaction using specific primers. The 3'UTR of six different isolates was sequenced following cloning and then submitted to NCBI. Phylogenetic analysis based on nucleotide sequences of the 3'UTR region showed that the entire world isolates could be classified into two main groups II and I and each one was divided into two subgroups. Isolates of this study were grouped with 23 isolates from Iran in subgroup IA and Four isolates from Khorasan province were placed in the IB subgroup. Four Iranian BBSV isolates from the West were grouped in the subgroup GIIA. All Chinese isolates and the European isolates together with the USA isolate clustered in subgroup GIIB. The results of nucleotide comparison of six isolates showed that although no mutation occurred at position 3477 (uridine instead of guanine), However, in position 3398, adenine replaced guanine (similar to 60% of Iranian isolates) and in position 3393, uridine was substituted for cytosine only in Fariman isolates (MW274750). The results of comparing the average nucleotide identity of 3'UTR isolates in this study showed that they are identical to Iranian isolates in the range from 97.75% to 97.98%. Nevertheless, they had the least identity with Kermanshah isolates (92.52%).

کلیدواژه‌ها [English]

  • "Plant viruses"
  • "sugar beet diseases"
  • "sequencing"
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