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نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی دانشگاه سیستان و بلوچستان

2 2. عضو هیات علمی (استادیار) بخش گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

10.22059/ijpps.2022.337876.1006995

چکیده

سوسک چهار نقطه‌ای حبوبات (Coleoptera: Chrysomelidae)(Fabricius, 1775) Callosobruchus maculatus به دلیل داشتن چرخه زندگی کوتاه و امکان پرورش آسان به عنوان حشره مدل برای مطالعه جنبه های مختلف زیست شناسی، رفتار شناسی و یا رقابت درون گونه‌ای استفاده می شود. در این مطالعه، با استفاده از لوبیاهای با یک تخم، تاثیر حضور لاروهای دیگر در لوبیاهای مجاور و نه در همان لوبیا در طول دوره رشدی، بر احساس رقابت احتمالی در حشره و رفتارهای جفت‌گیری در بزرگسالی و پارامترهای زیستی در نسل F0 و F1 بررسی شده است. طول دوره رشدی، تعداد تخم، درصد تفریخ، طول عمر حشره کامل، و نسبت جنسی نسل بعد ارزیابی شد. نتایج نشان داد لاروهای سوسک چهار نقطه‌ای حبوبات قادر بودند وجود لارو در لوبیاهایی که در تماس با لوبیاهای آنها بودند را تشخیص داده و تحت تاثیر آن در بزرگسالی تغییر رفتار نشان دهند. به طوری که این حشرات در بزرگسالی درصد جفت‌گیری موفق و مدت زمان جفت‌گیری کمتری نسبت به حشراتی که به تنهایی در یک ظرف پرورش یافته بودند را داشتند و وجود هیچ رقیبی را حس نکرده بودند. دیگر پارامترهای زیستی مطالعه شده (مانند تاخیر در جفت‌گیری و مدت زمان لگد زدن)، تحت تاثیر تیمارها تغییر معنی داری پیدا نکرد. بنابراین شرایط پرورش حشره (فقط وجود یک لوبیا با یک لارو در داخل آن در یک ظرف یا تراکمی از چند لوبیا به همراه تک لارو داخل هر کدام)، اثر معنی داری بر رفتارهای جفت‌گیری حشره در بزرگسالی میگذارد. این امر در طراحی آزمایشهای مختلف باید در نظر گرفته شود.

کلیدواژه‌ها

عنوان مقاله [English]

The study of effect of infected bean density in larval stage on biological parameters and mating behaviors of the cowpea weevil Callosobruchus maculatus

نویسندگان [English]

  • Azam Amiri 1
  • Faezeh Bagheri 2

2 Shiraz University2. Faculty member (Assistant Professor), Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran

چکیده [English]

The cowpea weevil Callosobruchus maculatus (Fabricius, 1775) (Coleoptera: Chrysomelidae) is used as a model insect to study various aspects of biology, behavior, or intraspecific competition due to its short life cycle and ease of breeding. In this study, using beans with an egg, the effect of other larvae in adjacent beans and not in the same bean during the growth period has been investigated on the possible feeling of competition in the insect and mating behaviors in adulthood and biological parameters in the F0 and F1 generations. Developmental time, the number of eggs, hatching percentage, adult longevity, and the sex ratio of the next generation were assessed. The results showed that the larvae of the cowpea weevil were able to detect the presence of larvae in the beans that were in contact with their beans, and they changed their behavior in adulthood under its influence. In adulthood, these insects had a successful mating percentage, and a shorter copulation duration than insects raised alone in a container and did not feel the presence of any competitors. Other studied biological parameters (such as mating latency and kick duration) did not change significantly under the influence of treatments. Therefore, insect breeding conditions (the presence of only one bean with one larva inside it in a container or compaction of several beans with a single larva inside each) significantly affect insect mating behaviors in adulthood. This should be considered in the design of various experiments.

کلیدواژه‌ها [English]

  • Fecundity
  • Competition
  • Cowpea weevil
  • Longevity
  • Copulation
  1. Afsheen, S., Wang, X., Li, R., Zhu, C.S. & Lou Y.G. (2008). Differential attraction of parasitoids in relation to specificity of kairomones from herbivores and their by-products. Insect Science, 15, 381-397.
  2. Amiri, A. & Bandani, A.R. (2021). Parents' living conditions influence offspring fitness and competency. Journal of Stored Products Research, 92, 101795.
  3. Arnqvist, G. & Tuda, M. (2010). Sexual conflict and the gender load: correlated evolution between population fitness and sexual dimorphism in seed beetles. Proceedings of the Royal Society of London Series B: Biological Sciences, 277, 1345-1352.
  4. Boivin, G. & Brodeur, J. (2006). Intra-and interspecific interactions among parasitoids: mechanisms, outcomes and biological control. In J. Brodeur & G. Boivin (ED.), Progress in Biological Control: Trophic and Guild Interactions in Biological Control (pp. 123–144). Springer, Dordretch, The Netherlands.
  5. Byrne, M. et al. (2009). Temperature, but not pH, compromises sea urchin fertilization and early development under near-future climate change scenarios. Proceedings of the Royal Society B:Biological Sciences, 276, 1883–1888.
  6. Edvardsson, M. & Canal, D. (2006). The effects of copulation duration in the bruchid beetle Callosobruchus maculatus. Behavioral Ecology, 17, 430–434.
  7. Goubault, M., Outreman, Y., Poinsot, D. & Cortesero, A.M. (2005). Patch exploitation strategies of parasitic wasps under intraspecific competition. Behavioral Ecology, 16, 693–701.
  8. Guedes, R.N.C., Guedes, N.M.P. & Smith, R.H. (2007). Larval competition within seeds: From the behaviour process to the ecological outcome in the seed beetle Callosobruchus maculatus. Austral Ecology, 32(6), 697-707.
  9. Han, C.S. & Brooks, R.C. (2015). The interaction between genotype and juvenile and adult density environment in shaping multidimensional reaction norms of behaviour. Functional Ecology, 29, 78-87.
  10. Harrison, X.A. et al. (2018). A brief introduction to mixed effects modelling and multi-model inference in ecology. PeerJ, 6, e4794.
  11. Iglesias-Carrasco, M., Brookes, S., Kruuk, L.E.B. & Head, M.L. (2020). The effects of competition on fitness depend on the sex of both competitors. Ecology and Evolution, 10(18), 9808-9826.
  12. Ishii, Y. & Shimada, M. (2008). Competitive exclusion between contest and scramble strategists in Callosobruchus seed–beetle modeling. Population Ecology, 50, 197-205.
  13. Kishi, S. (2015). Reproductive interference in laboratory experiments of interspecific competition. Population Ecology, 57, 283–292.
  14. Kyogoku, D. (2021). Seed beetles as a modern model system of interspecific competition. Ecologica research, 36(4), 580-589.
  15. Legros, M., Lloyd, A.L., Huang, Y. & Gould, F. (2009). Density-Dependent Intraspecific Competition in the Larval Stage of Aedes aegypti (Diptera: Culicidae): Revisiting the Current Paradigm. Journal of Medical Entomology, 46, 409–419.
  16. Małek, D.K. & Czarnoleski, M. (2021). Thermal Preferences of Cowpea Seed Beetles (Callosobruchus maculatus): Effects of Sex and Nuptial Gift Transfers. Insects, 12, 310.
  17. Messina, F.J., Bloxham, A.J. & Seargent, A.J. (2007). Mating compatibility between geographic populations of the seed beetle Callosobruchus maculatus. Journal of Insect Behavior, 20, 489501.
  18. Miyatake, T. & Matsumura, F. (2004). Intra-specific variation in female remating in Callosobruchus chinensis and maculatus. Journal of insect physiology, 50, 403–408.
  19. Robert, F.A., Brodeur, J. & Boivin, G. (2016). Patch exploitation strategies of parasitoids under indirect intra- and inter-specific competition. Ecological Entomology, 41, 590-598.
  20. Schade, D.J. & Vamosi, S.M. (2012). Larval competition reduces body condition in the female seed beetle, Callosobruchus maculatus. Journal of Insect Science, 12(1), 35.
  21. Simmons, L.W. (2001). Sperm competition and its evolutionary consequences in the insects. Princeton, New Jersey, Princeton University Press.
  22. Tirindelli, R., Dibattista, M., Pifferi, S. & Menini, A. (2009). From Pheromones to Behavior. Physiological Reviews. 89, 921-956.
  23. Thornton, A. (2008). Early body condition, time budgets and the acquisition of foraging skills in meerkats. Animal Behavior, 75, 951-962.
  24. Vamosi, S.M. (2005). Interactive effects of larval host and competition on adult fitness: an experimental test with seed beetles (Coleoptera: Bruchidae). Functional ecology, 19, 859–864.
  25. Vamosi, S.M. & Lesack, T.L. (2007). Direct effects of larval competition on development time and fecundity in seed beetles. Evolutionary ecology research, 9, 1131-1143.
  26. Wilson, C.J., Buzatto, B.A., Robinson, S.P. & Tomkins, J.L. (2014). Sociosexual environment influences patterns of ejaculate transfer and female kicking in Callosobruchus maculatus. Animal Behaviour, 94, 37-43.