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تأثیر اسیدیته و فشار اسمزی بر تولید افلاتوکسین در قارچ Aspergillus parasiticus

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاه‎پزشکی، دانشکدۀ کشاورزی، دانشگاه لرستان، ایران

2 دانشیار گروه گیاه‎پزشکی، دانشکدۀ کشاورزی، دانشگاه لرستان، ایران

3 استادیار، گروه گیاه‎پزشکی، دانشکدۀ کشاورزی، دانشگاه لرستان، ایران

چکیده

قارچ Aspergillus parasiticus با تولید افلاتوکسین B1تأثیرات جبران‌ناپذیری بر مصرف‌کنندگان مواد غذایی آلوده به این زهرابۀ قارچی می‌گذارد. در این پژوهش، تیمارهای مختلف pH و غلظت NaCl در محیط کشت مایع قارچ اثر داده شدند و توکسین‎زایی آنها با استفاده از سه روش محیط کشت نارگیل - آگار، TLC و HPLC بررسی و مشخص شد که افلاتوکسین‎های تولیدشده به‌وسیلۀ این قارچ، زیر نور UV خاصیت فلورسانس دارند. بیشترین توکسین‎زایی و رشد را در تیمار اسیدیته به‌ترتیب در 6 و 5 و در تیمار NaCl با غلظت 3 و صفر درصد شاهد بودیم. غلظت‎های بالای نمک باعث جلوگیری از رشد و به‌ تبع آن، تولید توکسین آن می‎شود. افلاتوکسین B1 بیشترین توکسین تولیدی این قارچ است، بنابراین می‎توان با کنترل این دو عامل از رشد و تولید افلاتوکسین این قارچ تا حدود زیادی پیشگیری کرد و از تأثیرات سوء آن جلوگیری به‎ عمل آورد.
 
 
 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of pH and NaCl on the aflatoxin production of Aspergillus parasiticus

نویسندگان [English]

  • Seyed Moslem Moosavian 1
  • Mostafa Darvishnia 2
  • Aeidi bazgir 3
1 M.Sc. Student of Plant Pathology, Assistant Department of Plant Protection, Faculty of Agriculture, Lorestan University, Iran
2 Accociate Professors, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Iran
3 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Iran
چکیده [English]

Aspergillus parasiticus causes adverse effects on its consumers of food infected with this fungal toxins by producing aflatoxin B1. In this study, the effects of different treatments of pH and NaCl concentration on the growth and toxin production of this fungus was investigated using three methods including coconut-agar medium, TLC, and HPLC. Based on our examinations, the aflatoxins produced by this fungus had fluorescence effect under UV light. The highest growth and production toxin of A. parasiticus was, respectively, occurred at pH 6 and 5 and NaCl concentration of 3 and 0 percent. High concentrations of salt suppressed this fungi growth and consequently its toxin production. Aflatoxin B1 was the most common toxin produced by A. parasiticus. As a result, by controlling these two factors, the growth and aflatoxin production of this fungus can be largely inhibited to avoid its adverse effects.
 
 

کلیدواژه‌ها [English]

  • Aspergillus
  • Toxin
  • pH
  • NaCl
 
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