c3518cb17d976b8

تاثیر توام تریپتوفان و اسید آسکوربیک بر برخی از فراسنجه های فیزیولوژیکی، زیستی و رفتاری کارگران زنبور عسل ایرانی Apis mellifaera meda L.

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد حشره شناسی کشاورزی

2 گروه گیاهپزشکی، دانشگاه زنجان، هیئت علمی

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی، کرج ایران

4 استادیار گروه علوم دامی، دانشکده کشاورزی دانشگاه آزاد اسلامی واحد کرج، کرج. ایران

چکیده

این مطالعه به منظور بررسی تاثیر تغذیه همزمان تریپتوفان و اسید اسکوربیک بر برخی از پارامترهای فیزیولوژیکی، رفتاری ، زیستی و تولیدی در زنبور عسل ایرانیApis mellifera L. انجام شد. تیمارهای انتخابی شامل تریپتوفان(12mg/gr)، اسید آسکوربیک(2mg/gr)، مخلوط هر دو و تیمار شاهد در قالب شربت 1:1 (1 کیلو گرم شکر در 1 لیتر آب) که هفته ای یکبار در اختیار زنبورها قرار گرفت. نتایج بررسی ها نشان داد تغذیه زنبورها با رژیم غذایی حاوی تریپتوفان و اسید آسکوربیک بر میزان مالون دی آلدهید(MDA)، ملاتونین(MEL) و سرتونین(SER) تاثیر معنی داری داشته است (P<0.01). بیشترین مقدار MDA ، MEL و SER بترتیب در تیمارهای تریپتوفان pg/ml) 72.0 ± 59.31 (، مخلوط تریپتوفان و اسید آسکوربیک(178±5.02 pg/ml) و اسید آسکوربیک (28.31±0.5 pg/ml)مشاهده شد. همچنین کمترین مقادیر MDA ، MEL وSER به ترتیب در تیمارهای اسید آسکوربیک 36 pg/ml).0±59.17(، ) (74±3.06 pg/ml و تریپتوفان (15.2±0.31 pg/ml) اندازه گیری شد. تفاوت وزنی زنبورهای تیمار شده بطور معنی داری متفاوت بود (P<0.001). سنگین ترین و سبک ترین زنبورها بترتیب در تیمار مخلوط تریپتوفان و اسید آسکوربیکmg) 26.3 ± 26.111( و شاهدmg) 75.1± 34.90( مشاهده شد. نتایج حاصل از واکنش زنبورها نسبت به گوی چرمی ( بعنوان واکنش دفاعی) نشان از عدم تاثیر معنی دار تیمارهای غذایی بر رفتار دفاعی زنبورهای کارگر داشت. رژیم های غذایی بر آرامش زنبورهای درون کلنی (P<0.05) و روی رفتار تهاجمی زنبورها تاثیر معنی داری داشته است(P<0.05) . تیمارهای غذایی بر جمعیت های تخم، لارو ، شفیره و تولید عسل تاثیر قابل توجهی را در مقایسه با شاهد داشته است ((P<0.001 .

کلیدواژه‌ها

عنوان مقاله [English]

Combined effects of edible tryptophan and ascorbic acid on some physiological, biological and behavioral parameters on workers of the Iranian honey bee, Apis mellifera meda L.

نویسندگان [English]

  • Ali Hamrahi 1
  • morteza movahedi fazel 2
  • mariam moarefi 3
  • nima eila 4
1 Dep. of plant protection, university of zanjan
3 Dep. of Agronomy and plant breeding, karaj branch, Islamic azad univ.
4 Assisstant Professor Department of Animal Science, College of Agriculture. Islamic Azad University-Karaj Branch. Karaj. Iran.
چکیده [English]

This study was considered the effect of bees feeding with mixture of tryptophan and ascorbic acid on some of physiological , behavioral, and productive parameters in Iranian honey bee. The treatments included tryptophan(12 mg/gr) , ascorbic acid (2 mg/gr) combined in 1:1 syrup ( sugar 1 kg per 1 liter of water), and for control treatment only used syrup. Bees were fed once a week.
The results showed that feeding bees with syrup contain tryptophan and ascorbic acid had significant effect on malondialdehyde(MDA), melatonin(MEL) and serotonin(SER) levels (P<0.01). The maximum levels of MDA, MEL and SER was observed in worker bees which they fed respectively with tryptophan (31.59± 0.72 pg/ml), combined tryptophan and ascorbic acid (178 ± 5.02 pg/ml) and ascorbic acid treatment (28.31± 5.02 pg/ml). The minimum levels was recorded respectively in ascorbic acid (17.59± 0.35 pg/ml), (74±3.06 pg/ml) and tryptophan(15.2±0.31 pg/ml) treatments. The weight of treated bees was affected statistically (P<0.001). The maximum weight was related to feeding bees with combine tryptophan and ascorbic acid syrup (111.26 ± 3.26 mg) and the minimum was in control group (90.34 ± 1.75 mg). The reaction of bees to the leather ball showed that dietary treatments had no effect on defensive behavior of worker bees. The defined diets had a significant effect on the peace of bees within the colony (P<0.05) and also on the offensive behavior of the bees (P<0.05). All treatments had significant effects on fecundity, larvae and pupae density and honey production in comparison with control (P<0.001).

کلیدواژه‌ها [English]

  • Malondialdehyde
  • Melatonin
  • Serotonin
  • Nutrition

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دانش گیاهپزشکی ایران
دوره 53، شماره 1
اردیبهشت 1401
صفحه 11-26

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سابقه مقاله

  • تاریخ دریافت: 28 تیر 1400
  • تاریخ بازنگری: 16 دی 1400
  • تاریخ پذیرش: 27 دی 1400

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